Generation of LTR-HSCs from 8.5 dpc P-Sp and YS (2 embryo equivalents) in coculture with AGM-S3 cells
| Cell source . | Culture condition . | No. of mice reconstituted/ transplanted . | % donor cells in reconstituted mice expressing . | ||
|---|---|---|---|---|---|
| B220 . | Thy-1 . | Gr-1/Mac-1 . | |||
| None | AGM-S3 cells | 0/8 | NA | NA | NA |
| P-Sp | Before culture | 0/9 | NA | NA | NA |
| Without stroma | 0/4 | NA | NA | NA | |
| AGM-S3 cells | 4/4 | 66.2 | 46.0 | 32.7 | |
| YS | Before culture | 0/9 | NA | NA | NA |
| Without stroma | 0/4 | NA | NA | NA | |
| AGM-S3 cells | 4/4 | 74.1 | 55.6 | 36.3 | |
| Cell source . | Culture condition . | No. of mice reconstituted/ transplanted . | % donor cells in reconstituted mice expressing . | ||
|---|---|---|---|---|---|
| B220 . | Thy-1 . | Gr-1/Mac-1 . | |||
| None | AGM-S3 cells | 0/8 | NA | NA | NA |
| P-Sp | Before culture | 0/9 | NA | NA | NA |
| Without stroma | 0/4 | NA | NA | NA | |
| AGM-S3 cells | 4/4 | 66.2 | 46.0 | 32.7 | |
| YS | Before culture | 0/9 | NA | NA | NA |
| Without stroma | 0/4 | NA | NA | NA | |
| AGM-S3 cells | 4/4 | 74.1 | 55.6 | 36.3 | |
Cells isolated from 8.5 dpc P-Sp and YS of Ly-5.2 mice or their progenies were transplanted into Ly-5.1 mice, and 12 weeks later Ly-5.2+ B lymphocytes (B220+), T lymphocytes (Thy-1+), and myeloid cells (Gr-1+/Mac-1+) were analyzed by flow cytometry.
NA indicates not analyzed.