Generation of CFU-S from 8.5 dpc P-Sp and YS (one embryo equivalent) in coculture with AGM-S3 cells
Cells injected
.
Before coculture
.
After coculture
.
Day 1
.
Day 2
.
Day 3
.
Day 4
.
None
0
0
0
0
0
P-Sp
0
0
0
0
7 ± 3 (n = 10)
YS
0
0
0
0
3 ± 2 (n = 6)
Cells injected
.
Before coculture
.
After coculture
.
Day 1
.
Day 2
.
Day 3
.
Day 4
.
None
0
0
0
0
0
P-Sp
0
0
0
0
7 ± 3 (n = 10)
YS
0
0
0
0
3 ± 2 (n = 6)
Cells isolated from 8.5 dpc P-Sp and YS or their progenies cocultured with AGM-S3 cells were given intravenously to mice, and 8 days later the number of colonies in spleens of recipients was counted.