Polymerase chain reaction (PCR) and reverse transcription–PCR primer sequences
| Primers . | 5′ to 3′ sequences . | Amplification bp . |
|---|---|---|
| PCR | ||
| Exon 1α | 340 | |
| Forward | GAA GAA AGA GGA GGG GCT G | |
| Reverse | GCG CTA CCT GAT TCC AAT TC | |
| Exon 2 | 394 | |
| Forward | TTC CTT TCC GTC ATG CCG G | |
| Reverse | GTA CAA ATT CTC AGA TCA TCA GTC GTC | |
| Exon 3 | 323 | |
| Forward | GAC CTG GAG CGC TTG AGC GG | |
| Reverse | GTG GCC CTG TAG GAC CTT CGG | |
| Exon 1β | 440 | |
| Forward | TCC CAG TCT GAC GTT AAG GGG | |
| Reverse | GTC TAA GTC GTT GTA ACC CG | |
| RT-PCR | ||
| INK4a (exon 1α to exon 3) | 494 | |
| Forward | ATG GAG CCT TCG GCT GAC TGG C | |
| Reverse | GAC TGA TGA TCT AAG TTT CCC GAG G | |
| ARF (exon 1β to exon 2) | 571 | |
| Forward | GAG TGG CGC TGC TCA CCT CTG G | |
| Reverse | GGG ACC TTC CGC GGC ATC | |
| INK4a | 142 | |
| Forward | ATG GAG CCT TCG GCT GAC TGG C | |
| Reverse | CTG CCC ATC ATC ATG ACC TGG A | |
| ARF | 212 | |
| Forward | CAT GGT GCG CAG GTT CTT GG | |
| Reverse | same as INK4a |
| Primers . | 5′ to 3′ sequences . | Amplification bp . |
|---|---|---|
| PCR | ||
| Exon 1α | 340 | |
| Forward | GAA GAA AGA GGA GGG GCT G | |
| Reverse | GCG CTA CCT GAT TCC AAT TC | |
| Exon 2 | 394 | |
| Forward | TTC CTT TCC GTC ATG CCG G | |
| Reverse | GTA CAA ATT CTC AGA TCA TCA GTC GTC | |
| Exon 3 | 323 | |
| Forward | GAC CTG GAG CGC TTG AGC GG | |
| Reverse | GTG GCC CTG TAG GAC CTT CGG | |
| Exon 1β | 440 | |
| Forward | TCC CAG TCT GAC GTT AAG GGG | |
| Reverse | GTC TAA GTC GTT GTA ACC CG | |
| RT-PCR | ||
| INK4a (exon 1α to exon 3) | 494 | |
| Forward | ATG GAG CCT TCG GCT GAC TGG C | |
| Reverse | GAC TGA TGA TCT AAG TTT CCC GAG G | |
| ARF (exon 1β to exon 2) | 571 | |
| Forward | GAG TGG CGC TGC TCA CCT CTG G | |
| Reverse | GGG ACC TTC CGC GGC ATC | |
| INK4a | 142 | |
| Forward | ATG GAG CCT TCG GCT GAC TGG C | |
| Reverse | CTG CCC ATC ATC ATG ACC TGG A | |
| ARF | 212 | |
| Forward | CAT GGT GCG CAG GTT CTT GG | |
| Reverse | same as INK4a |
RT indicates reverse transcription; bp, base pair.