Vβ usage in T-cell lines and T-ALL samples as determined by molecular analysis and mAb reactivity
| Sample . | TCRβ chain . | Age, sex . | SB analysis . | RT-PCR + HD . | Sequence analysis of RT-PCR products . | Vβ MAb reactivity . | |
|---|---|---|---|---|---|---|---|
| Allele 1 (frame) . | Allele 2 (frame) . | ||||||
| T-cell lines | |||||||
| CEM | CyTCRβ | NA | R/R | Vβ9-Cβ | Vβ9-Jβ2.3 (+) | — | Vβ9.1 |
| DND-41 | TCRβδ+ | NA | R/R | Vβ13-Cβ/Vβ18-Cβ | Vβ18-Jβ1.2 (+) | Vβ13-Jβ2.7 (−) | no reactivity |
| HPB-ALL | TCRαβ+ | NA | R/R | Vβ5-Cβ/Vβ6-Cβ | Vβ5-Jβ2.5 (+) | Vβ6-Jβ2.5 (−) | Vβ5.2/5.3 |
| HSB-2 | CyTCRβ | NA | R/R | Vβ5-Cβ | Vβ5-Jβ1.1 (+) | — | Vβ5.1 |
| HUT78/H9 | TCRαβ+ | NA | R/R | Vβ23-Cβ | Vβ23-Jβ1.2 (+) | — | Vβ23 |
| KT-1 | TCRαβ+ | NA | R/R | Vβ15-Cβ/Vβ18-Cβ | Vβ18-Jβ2.3 (+) | Vβ15-Jβ1.2 (+) | Vβ18 |
| MOLT16/17 | TCRαβ+ | NA | R/R | Vβ2-Cβ | Vβ2-Jβ2.3 (+) | — | Vβ2 |
| MOLT3/4 | CyTCRβ | NA | R/R | Vβ2-Cβ/Vβ12-Cβ | Vβ2-Jβ2.1 (+) | Vβ12-Jβ2.5 (−) | Vβ2 |
| P12 | CyTCRβ | NA | R/R | Vβ13-Cβ | Vβ13-Jβ2.1 (+) | — | Vβ13.1/13.3 |
| PF-382 | CyTCRβ | NA | R/R | Vβ2-Cβ/Vβ6-Cβ | Vβ2-Jβ2.1 (+) | Vβ6-Jβ2.7 (−) | Vβ2 |
| RPMI8402 | CyTCRβ | NA | R/R | Vβ17-Cβ/Vβ24-Cβ | Vβ24-Jβ1.5 (+) | Vβ17-Jβ2.7 (−) | no reactivity |
| SUP-T3 | CyTCRβ | NA | R/R | Vβ3-Cβ | Vβ3-Jβ2.3 (+) | — | Vβ3 |
| T-ALL samples | |||||||
| T002 | TCRαβ+T-ALL | 5, F | R/R | Vβ3-Cβ | Vβ3-Jβ1.5 (+) | — | Vβ3 |
| T010 | TCRαβ+T-ALL | 3, M | R/R | Vβ7-Cβ | Vβ7-Jβ1.4 (+) | — | no reactivity |
| T012 | TCRαβ+ T-ALL | 34, M | R/R | Vβ9-Cβ/Vβ11-Cβ/Vβ23Cβ/Vβ24-Cβ3-150 | Vβ11-Jβ1.5 (+)/Vβ24-Jβ1.5 (+) | Vβ9-Jβ2.1 (+)/Vβ23-Jβ1.2 (+) | no reactivity |
| T015 | TCRαβ+ T-ALL | np, np | R/R | Vβ4-Cβ/Vβ6-Cβ | Vβ4-Jβ2.1 (+) | Vβ6-Jβ1.1 (−) | no reactivity |
| T017 | TCRαβ+ T-ALL | 3, F | R/R | Vβ13-Cβ/Vβ20-Cβ | Vβ20-Jβ1.5 (+) | Vβ13-Jβ1.2 (−) | Vβ20 |
| T020 | TCRαβ+ T-ALL | 14, np | R/R | Vβ7-Cβ/Vβ21-Cβ | Vβ21-Jβ2.3 (+) | Vβ7-Jβ2.2 (−) | Vβ21.3 |
| T029 | TCRαβ+ T-ALL | 10, F | R/R | Vβ22-Cβ | Vβ22-Jβ1.1 (+) | — | Vβ22 |
| T044 | TCRαβ+T-ALL | np, np | R/R | Vβ6-Cβ/Vβ21-Cβ/Vβ24-Cβ3-150 | Vβ6-Jβ1.2 (+)/Vβ21-Jβ2.1 (+) | Vβ24-Jβ1.1 (+) | no reactivity |
| T047 | TCRαβ+ T-ALL | np, np | R/R | Vβ2-Cβ | Vβ2-Jβ1.2 (+) | — | Vβ2 |
| T069 | TCRαβ+ T-ALL | 29, M | R/R | Vβ3-Cβ | Vβ3-Jβ1.6 (+) | — | Vβ3 |
| T077 | TCRαβ+ T-ALL | 10, M | R/R | Vβ3-Cβ/Vβ8-Cβ | Vβ8-Jβ2.1 (+) | Vβ3-Jβ2.1 (+) | Vβ8 |
| T139 | TCRαβ+ T-ALL | 14, M | R/R | Vβ3-Cβ/Vβ11-Cβ | Vβ3-Jβ2.1 (+) | Vβ11-Jβ1.1 (+) | no reactivity |
| T140 | TCRαβ+ T-ALL | 28, M | R/R | Vβ3-Cβ/Vβ6-Cβ | Vβ3-Jβ2.5 (+) | Vβ6-Jβ1.2 (+) | Vβ3 |
| T145 | TCRαβ+ T-ALL | 51, M | R/R | Vβ5-Cβ/Vβ7-Cβ | Vβ5-Jβ1.5 (+) | Vβ7-Jβ2.3 (+) | no reactivity |
| T181 | TCRαβ+ T-ALL | np, np | R/R | Vβ7-Cβ | Vβ7-Jβ2.1 (+) | — | no reactivity |
| T182 | TCRαβ+ T-ALL | np, np | R/R | Vβ6-Cβ/Vβ13-Cβ | Vβ6-Jβ1.1 (+)3-151 | Vβ13-Jβ2.5 (−) | no reactivity |
| Sample . | TCRβ chain . | Age, sex . | SB analysis . | RT-PCR + HD . | Sequence analysis of RT-PCR products . | Vβ MAb reactivity . | |
|---|---|---|---|---|---|---|---|
| Allele 1 (frame) . | Allele 2 (frame) . | ||||||
| T-cell lines | |||||||
| CEM | CyTCRβ | NA | R/R | Vβ9-Cβ | Vβ9-Jβ2.3 (+) | — | Vβ9.1 |
| DND-41 | TCRβδ+ | NA | R/R | Vβ13-Cβ/Vβ18-Cβ | Vβ18-Jβ1.2 (+) | Vβ13-Jβ2.7 (−) | no reactivity |
| HPB-ALL | TCRαβ+ | NA | R/R | Vβ5-Cβ/Vβ6-Cβ | Vβ5-Jβ2.5 (+) | Vβ6-Jβ2.5 (−) | Vβ5.2/5.3 |
| HSB-2 | CyTCRβ | NA | R/R | Vβ5-Cβ | Vβ5-Jβ1.1 (+) | — | Vβ5.1 |
| HUT78/H9 | TCRαβ+ | NA | R/R | Vβ23-Cβ | Vβ23-Jβ1.2 (+) | — | Vβ23 |
| KT-1 | TCRαβ+ | NA | R/R | Vβ15-Cβ/Vβ18-Cβ | Vβ18-Jβ2.3 (+) | Vβ15-Jβ1.2 (+) | Vβ18 |
| MOLT16/17 | TCRαβ+ | NA | R/R | Vβ2-Cβ | Vβ2-Jβ2.3 (+) | — | Vβ2 |
| MOLT3/4 | CyTCRβ | NA | R/R | Vβ2-Cβ/Vβ12-Cβ | Vβ2-Jβ2.1 (+) | Vβ12-Jβ2.5 (−) | Vβ2 |
| P12 | CyTCRβ | NA | R/R | Vβ13-Cβ | Vβ13-Jβ2.1 (+) | — | Vβ13.1/13.3 |
| PF-382 | CyTCRβ | NA | R/R | Vβ2-Cβ/Vβ6-Cβ | Vβ2-Jβ2.1 (+) | Vβ6-Jβ2.7 (−) | Vβ2 |
| RPMI8402 | CyTCRβ | NA | R/R | Vβ17-Cβ/Vβ24-Cβ | Vβ24-Jβ1.5 (+) | Vβ17-Jβ2.7 (−) | no reactivity |
| SUP-T3 | CyTCRβ | NA | R/R | Vβ3-Cβ | Vβ3-Jβ2.3 (+) | — | Vβ3 |
| T-ALL samples | |||||||
| T002 | TCRαβ+T-ALL | 5, F | R/R | Vβ3-Cβ | Vβ3-Jβ1.5 (+) | — | Vβ3 |
| T010 | TCRαβ+T-ALL | 3, M | R/R | Vβ7-Cβ | Vβ7-Jβ1.4 (+) | — | no reactivity |
| T012 | TCRαβ+ T-ALL | 34, M | R/R | Vβ9-Cβ/Vβ11-Cβ/Vβ23Cβ/Vβ24-Cβ3-150 | Vβ11-Jβ1.5 (+)/Vβ24-Jβ1.5 (+) | Vβ9-Jβ2.1 (+)/Vβ23-Jβ1.2 (+) | no reactivity |
| T015 | TCRαβ+ T-ALL | np, np | R/R | Vβ4-Cβ/Vβ6-Cβ | Vβ4-Jβ2.1 (+) | Vβ6-Jβ1.1 (−) | no reactivity |
| T017 | TCRαβ+ T-ALL | 3, F | R/R | Vβ13-Cβ/Vβ20-Cβ | Vβ20-Jβ1.5 (+) | Vβ13-Jβ1.2 (−) | Vβ20 |
| T020 | TCRαβ+ T-ALL | 14, np | R/R | Vβ7-Cβ/Vβ21-Cβ | Vβ21-Jβ2.3 (+) | Vβ7-Jβ2.2 (−) | Vβ21.3 |
| T029 | TCRαβ+ T-ALL | 10, F | R/R | Vβ22-Cβ | Vβ22-Jβ1.1 (+) | — | Vβ22 |
| T044 | TCRαβ+T-ALL | np, np | R/R | Vβ6-Cβ/Vβ21-Cβ/Vβ24-Cβ3-150 | Vβ6-Jβ1.2 (+)/Vβ21-Jβ2.1 (+) | Vβ24-Jβ1.1 (+) | no reactivity |
| T047 | TCRαβ+ T-ALL | np, np | R/R | Vβ2-Cβ | Vβ2-Jβ1.2 (+) | — | Vβ2 |
| T069 | TCRαβ+ T-ALL | 29, M | R/R | Vβ3-Cβ | Vβ3-Jβ1.6 (+) | — | Vβ3 |
| T077 | TCRαβ+ T-ALL | 10, M | R/R | Vβ3-Cβ/Vβ8-Cβ | Vβ8-Jβ2.1 (+) | Vβ3-Jβ2.1 (+) | Vβ8 |
| T139 | TCRαβ+ T-ALL | 14, M | R/R | Vβ3-Cβ/Vβ11-Cβ | Vβ3-Jβ2.1 (+) | Vβ11-Jβ1.1 (+) | no reactivity |
| T140 | TCRαβ+ T-ALL | 28, M | R/R | Vβ3-Cβ/Vβ6-Cβ | Vβ3-Jβ2.5 (+) | Vβ6-Jβ1.2 (+) | Vβ3 |
| T145 | TCRαβ+ T-ALL | 51, M | R/R | Vβ5-Cβ/Vβ7-Cβ | Vβ5-Jβ1.5 (+) | Vβ7-Jβ2.3 (+) | no reactivity |
| T181 | TCRαβ+ T-ALL | np, np | R/R | Vβ7-Cβ | Vβ7-Jβ2.1 (+) | — | no reactivity |
| T182 | TCRαβ+ T-ALL | np, np | R/R | Vβ6-Cβ/Vβ13-Cβ | Vβ6-Jβ1.1 (+)3-151 | Vβ13-Jβ2.5 (−) | no reactivity |
Multiple Vβ-Cβ products probably resulting from subclone formation with one major clone and one minor clone (< 10%) not detectable by SB analysis.
Transcript involves Cβ2 segment coupled to the Jβ1.1 segment of the VDJ exon, which fits with deletion as seen in SB analysis. np indicates not provided.