Vβ antibodies and their reactivity in healthy controls
| Vβ antibody specificity . | mAb clone . | Manufacturer . | % Vβ expression per CD3/TCRαβ cells* . | |
|---|---|---|---|---|
| Median (p10-p90) . | Upper limit (3 SD) . | |||
| Vβ1 | BL37.2 | Immunotech | 4.1 (3.4-5.9) | 8.9 |
| Vβ2 | MPB2/D5 | Immunotech | 8.6 (6.2-11.2) | 14.0 |
| Vβ3 | CH92 | Immunotech | 4.5 (1.5-8.5) | 12.5 |
| Vβ5.1 | IMMU157 | Immunotech | 6.5 (4.0-8.7) | 12.3 |
| Vβ5.2/5.3 | 4H11 | T-cell Sciences | 3.2 (2.1-3.8) | 5.1 |
| Vβ6.1 | CRI304.3 | Immunotech | 1.3 (0.5-2.8) | 4.1 |
| Vβ6.7 | OT145 | T-cell Diagnostics | 3.0 (0.5-5.5) | 8.2 |
| Vβ7.1 | Zoé | Immunotech | 2.8 (1.6-4.6) | 6.4 |
| Vβ8.1/8.2 | 56C5.2 | Immunotech | 5.0 (3.5-6.5) | 8.6 |
| Vβ9.1 | FIN9 | Immunotech | 3.2 (2.1-4.7) | 7.2 |
| Vβ11.1 | C21 | Immunotech | 0.8 (0.5-1.4) | 2.2 |
| Vβ12.2 | VER2.32 | Immunotech | 2.4 (1.3-3.6) | 6.5 |
| Vβ13.1/13.3 | BAM13 | T-cell Sciences | 5.9 (4.3-10.8) | 20.5 |
| Vβ13.6 | JU74.3 | Immunotech | 2.1 (1.5-3.0) | 5.7 |
| Vβ14 | CAS1.1.3 | Immunotech | 3.7 (2.1-8.6) | 16.9 |
| Vβ16 | TAMAYA1.2 | Immunotech | 1.3 (1.0-1.7) | 2.2 |
| Vβ17 | E17.5F3 | Immunotech | 5.2 (3.8-6.5) | 8.9 |
| Vβ18 | BA62.6 | Immunotech | 0.5 (0.5-1.5) | 2.1 |
| Vβ20 | ELL1.4 | Immunotech | 2.4 (1.1-4.1) | 5.9 |
| Vβ21.3 | IG125 | Immunotech | 2.8 (1.8-4.2) | 6.9 |
| Vβ22 | IMMU546 | Immunotech | 4.0 (0.6-5.3) | 11.1 |
| Vβ23 | HUT78.7/AF23 | Immunotech | 1.3 (0.5-2.5) | 3.8 |
| Vβ antibody specificity . | mAb clone . | Manufacturer . | % Vβ expression per CD3/TCRαβ cells* . | |
|---|---|---|---|---|
| Median (p10-p90) . | Upper limit (3 SD) . | |||
| Vβ1 | BL37.2 | Immunotech | 4.1 (3.4-5.9) | 8.9 |
| Vβ2 | MPB2/D5 | Immunotech | 8.6 (6.2-11.2) | 14.0 |
| Vβ3 | CH92 | Immunotech | 4.5 (1.5-8.5) | 12.5 |
| Vβ5.1 | IMMU157 | Immunotech | 6.5 (4.0-8.7) | 12.3 |
| Vβ5.2/5.3 | 4H11 | T-cell Sciences | 3.2 (2.1-3.8) | 5.1 |
| Vβ6.1 | CRI304.3 | Immunotech | 1.3 (0.5-2.8) | 4.1 |
| Vβ6.7 | OT145 | T-cell Diagnostics | 3.0 (0.5-5.5) | 8.2 |
| Vβ7.1 | Zoé | Immunotech | 2.8 (1.6-4.6) | 6.4 |
| Vβ8.1/8.2 | 56C5.2 | Immunotech | 5.0 (3.5-6.5) | 8.6 |
| Vβ9.1 | FIN9 | Immunotech | 3.2 (2.1-4.7) | 7.2 |
| Vβ11.1 | C21 | Immunotech | 0.8 (0.5-1.4) | 2.2 |
| Vβ12.2 | VER2.32 | Immunotech | 2.4 (1.3-3.6) | 6.5 |
| Vβ13.1/13.3 | BAM13 | T-cell Sciences | 5.9 (4.3-10.8) | 20.5 |
| Vβ13.6 | JU74.3 | Immunotech | 2.1 (1.5-3.0) | 5.7 |
| Vβ14 | CAS1.1.3 | Immunotech | 3.7 (2.1-8.6) | 16.9 |
| Vβ16 | TAMAYA1.2 | Immunotech | 1.3 (1.0-1.7) | 2.2 |
| Vβ17 | E17.5F3 | Immunotech | 5.2 (3.8-6.5) | 8.9 |
| Vβ18 | BA62.6 | Immunotech | 0.5 (0.5-1.5) | 2.1 |
| Vβ20 | ELL1.4 | Immunotech | 2.4 (1.1-4.1) | 5.9 |
| Vβ21.3 | IG125 | Immunotech | 2.8 (1.8-4.2) | 6.9 |
| Vβ22 | IMMU546 | Immunotech | 4.0 (0.6-5.3) | 11.1 |
| Vβ23 | HUT78.7/AF23 | Immunotech | 1.3 (0.5-2.5) | 3.8 |
Reference values are derived from Van den Beemd et al.10