Loss of sensitivity of CFU-C to Fas cross-linking after depletion of mature or B220+ spleen cells
| . | CFU-C/106 cells . | ||
|---|---|---|---|
| Total spleen . | Lin− . | B220− . | |
| Medium | 48.3 ± 8.8 | 1133.3 ± 233.3 | 82.7 ± 8.9 |
| IFN-γ | 33.3 ± 4.4 | 1633.3 ± 438.3 | 135.0 ± 9.5 |
| Anti-Fas mAb | 40.0 ± 10.0 | 1533.3 ± 233.3 | 95.7 ± 4.9 |
| Anti-Fas mAb + IFN-γ | 16.7 ± 1.7* | 1100.0 ± 208.0NS | 110.0 ± 5.2NS |
| . | CFU-C/106 cells . | ||
|---|---|---|---|
| Total spleen . | Lin− . | B220− . | |
| Medium | 48.3 ± 8.8 | 1133.3 ± 233.3 | 82.7 ± 8.9 |
| IFN-γ | 33.3 ± 4.4 | 1633.3 ± 438.3 | 135.0 ± 9.5 |
| Anti-Fas mAb | 40.0 ± 10.0 | 1533.3 ± 233.3 | 95.7 ± 4.9 |
| Anti-Fas mAb + IFN-γ | 16.7 ± 1.7* | 1100.0 ± 208.0NS | 110.0 ± 5.2NS |
Total or magnetically depleted Lin− or B220− spleen cells were preincubated for 24 hours at a concentration of 107, 106, or 107cells/mL, respectively. Total and sorted cell populations were plated in a final volume of 1 mL MethoCult at concentrations ranging from 0.2 to 5 × 105 cells per culture dish and scored for colonies on day 7 or 8. Data represent means ± SEM from triplicate determinations of a typical experiment reproduced twice.
NS, not significant in comparison with controls.
P < .05.