Structural analysis of N-linked oligosaccharide chains purified from 6-19J and L8D immunoglobulin G3 monoclonal antibodies and from serum polyclonal immunoglobulin G of C3H mice
| IgG . | Sialylated glycoforms* . | Galactosylated glycoforms† . | |||||
|---|---|---|---|---|---|---|---|
| N (%) . | A1 (%) . | A2 (%) . | G0 (%) . | G1 (%) . | G2 (%) . | G1 + G2 G0 . | |
| 6-19J | 92.4 | 7.4 | 0.2 | 21.9 | 55.4 | 22.7 | 3.57 |
| L8D | 92.3 | 6.5 | 1.2 | 36.4 | 49.3 | 14.3 | 1.75 |
| C3H | 90.3 | 6.8 | 2.9 | 33.3 | 46.6 | 20.1 | 2.00 |
| IgG . | Sialylated glycoforms* . | Galactosylated glycoforms† . | |||||
|---|---|---|---|---|---|---|---|
| N (%) . | A1 (%) . | A2 (%) . | G0 (%) . | G1 (%) . | G2 (%) . | G1 + G2 G0 . | |
| 6-19J | 92.4 | 7.4 | 0.2 | 21.9 | 55.4 | 22.7 | 3.57 |
| L8D | 92.3 | 6.5 | 1.2 | 36.4 | 49.3 | 14.3 | 1.75 |
| C3H | 90.3 | 6.8 | 2.9 | 33.3 | 46.6 | 20.1 | 2.00 |
Molar ratios of neutral asialo (N), acidic monosialo (A1), and acidic disialo (A2) oligosaccharides of IgG3 mAb and polyclonal IgG purified from 4-month-old C3H mice.
Molar ratios of nongalactosylated (G0), monogalactosylated (G1), and digalactosylated (G2) glycoforms in desialylated oligosaccharides of monoclonal and polyclonal IgG.