Table 4. In vitro V(D)J recombination... | American Society of Hematology

Table 4.

In vitro V(D)J recombination assay with Vβ14–recombination signal sequences

	pHRecCJ		pH2V14CJ
RAG1	wt	wt	R973H	L732F	R404Q	wt	wt
RAG2	wt	wt	wt	wt	wt	R39G	R229Q
Blue col	270	9	0	0	0	0	0
Total	37 800	7800	34 500	20 700	88 800	156 000	117 000
R(× 10⁻²)^4-150	2.1	0.1	< 0.002	< 0.004	< 0.001	< 0.0006	< 0.0008

	pHRecCJ		pH2V14CJ
RAG1	wt	wt	R973H	L732F	R404Q	wt	wt
RAG2	wt	wt	wt	wt	wt	R39G	R229Q
Blue col	270	9	0	0	0	0	0
Total	37 800	7800	34 500	20 700	88 800	156 000	117 000
R(× 10⁻²)^4-150	2.1	0.1	< 0.002	< 0.004	< 0.001	< 0.0006	< 0.0008

wt indicates wild type.

R (coding joints) = 3 × (Blue col)/(Total) × 10.