Induction of redirected antileukemic T-cell cytotoxicity following stimulation with B7-1 immunoglobulin G-coated acute myeloid leukemia-M5 blasts in autologous mixed-lymphocyte tumor culture
| Plastic-bound OKT3 (ng per well) . | Specific lysis from AML-M5 blasts by remission T cells (%) stimulated with AML-M5 blasts according to pretreatment of blasts . | ||
|---|---|---|---|
| CD8 IgG . | B7-1(CD80) IgG . | P value* . | |
| 0 | 7.8 ± 3.1 | 5.0 ± 0.5 | — |
| 1 | 23.7 ± 1.0 | 26.3 ± 1.8 | 0.05 |
| 2 | 26.0 ± 0.2 | 30.0 ± 3.6 | 0.05 |
| 4 | 24.4 ± 1.6 | 28.5 ± 2.0 | 0.05 |
| Plastic-bound OKT3 (ng per well) . | Specific lysis from AML-M5 blasts by remission T cells (%) stimulated with AML-M5 blasts according to pretreatment of blasts . | ||
|---|---|---|---|
| CD8 IgG . | B7-1(CD80) IgG . | P value* . | |
| 0 | 7.8 ± 3.1 | 5.0 ± 0.5 | — |
| 1 | 23.7 ± 1.0 | 26.3 ± 1.8 | 0.05 |
| 2 | 26.0 ± 0.2 | 30.0 ± 3.6 | 0.05 |
| 4 | 24.4 ± 1.6 | 28.5 ± 2.0 | 0.05 |
Remission PHA blasts, 5 × 104 per well (activated for 3 days), of patient no. 39 with AML-M5a were stimulated with submitogenic concentrations of plastic-bound OKT3 as indicated, and with autologous mitomycin C-inactivated AML blasts (105per well) precoated with saturating concentrations of CD8 IgG and B7-1 IgG on day 0 and 7. Fusion cytokine binding was verified as stated in legend to Figure 3. On day 14 of mixed-lymphocyte tumor culture, cytotoxicity of responding T cells against autologous AML-M5 blasts (5 × 103 per well) in the presence of soluble OKT3 (80 ng/mL) was measured by standard 51Cr-release assay. Mean and SD of triplicate measurements are shown. Spontaneous51Cr-release was 18.2%.
AML indicates acute myloid leukemia; IgG, immunoglobulin G; PHA, phytohemagglutinin; 51Cr, 51chromium.
CD8 IgG-pretreated versus B7-1 IgG-pretreated stimulator cells compared by Wilcoxon test for unpaired rank sums.