Effect of the GHR mutation on CD36-binding characteristics
| Construct expressed . | Positive cells (%)3-150 . | No. beads per 100 positive cells3-151 . |
|---|---|---|
| ItG2 (DIE) CIDR-1 | 91.7 ± 3.1 | 5730 ± 128 |
| ItG2-CS2 (GHR) CIDR-1 | 1 ± 1 | — |
| MC CIDR-1 | 97.3 ± 1.5 | 7990 ± 263 |
| MC (GHR) CIDR-1 | 43.7 ± 4.5 | 2869 ± 188 |
| Construct expressed . | Positive cells (%)3-150 . | No. beads per 100 positive cells3-151 . |
|---|---|---|
| ItG2 (DIE) CIDR-1 | 91.7 ± 3.1 | 5730 ± 128 |
| ItG2-CS2 (GHR) CIDR-1 | 1 ± 1 | — |
| MC CIDR-1 | 97.3 ± 1.5 | 7990 ± 263 |
| MC (GHR) CIDR-1 | 43.7 ± 4.5 | 2869 ± 188 |
CIDR-1 constructs were transfected into COS-7 cells and tested for binding to CD36-coated Dynal beads.
For abbreviations, see Table 1.
See footnote (marked by an asterisk) to Table 1.
The number of beads was counted on 100 cells determined positive for binding to CD36-coated Dynal beads. Results are expressed as the mean and SD of 3 independent experiments.