Progenitor colony counts for methylcellulose assays
| Colony type . | Bone marrow . | |||||
|---|---|---|---|---|---|---|
| E . | G . | M . | GM . | GEMM . | Cells recovered (× 106) . | |
| PU.1+/+Spi-B+/+ | 1.6 ± 0.5 | 30.6 ± 6.4 | 37.4 ± 4.5 | 6.4 ± 1.3 | 19.9 ± 5.7 | 1.2 ± 0.6 |
| PU.1+/−Spi-B+/+ | 5.4 ± 1.3 | 57.4 ± 16.3 | 56.0 ± 8.3 | 8.0 ± 3.0 | 10.0 ± 2.3 | 2.8 ± 1.3 |
| PU.1+/−Spi-B+/− | 4.8 ± 1.5 | 56.3 ± 6.4 | 48 ± 5.0 | 6.5 ± 0.9 | 21.8 ± 3.6 | 1.3 ± 0.2 |
| PU.1+/+Spi-B−/− | 2 ± 0.7 | 28.9 ± 5.4 | 50.4 ± 9.4 | 11.1 ± 3.7 | 14.4 ± 2.1 | 0.8 ± 0.1 |
| PU.1+/−Spi-B−/− | 3.5 ± 2.0 | 38 ± 10.1 | 52.3 ± 7.8 | 3.3 ± 1.0 | 13.2 ± 4.0 | 0.4 ± 0.1 |
| Colony type . | Bone marrow . | |||||
|---|---|---|---|---|---|---|
| E . | G . | M . | GM . | GEMM . | Cells recovered (× 106) . | |
| PU.1+/+Spi-B+/+ | 1.6 ± 0.5 | 30.6 ± 6.4 | 37.4 ± 4.5 | 6.4 ± 1.3 | 19.9 ± 5.7 | 1.2 ± 0.6 |
| PU.1+/−Spi-B+/+ | 5.4 ± 1.3 | 57.4 ± 16.3 | 56.0 ± 8.3 | 8.0 ± 3.0 | 10.0 ± 2.3 | 2.8 ± 1.3 |
| PU.1+/−Spi-B+/− | 4.8 ± 1.5 | 56.3 ± 6.4 | 48 ± 5.0 | 6.5 ± 0.9 | 21.8 ± 3.6 | 1.3 ± 0.2 |
| PU.1+/+Spi-B−/− | 2 ± 0.7 | 28.9 ± 5.4 | 50.4 ± 9.4 | 11.1 ± 3.7 | 14.4 ± 2.1 | 0.8 ± 0.1 |
| PU.1+/−Spi-B−/− | 3.5 ± 2.0 | 38 ± 10.1 | 52.3 ± 7.8 | 3.3 ± 1.0 | 13.2 ± 4.0 | 0.4 ± 0.1 |
| Colony type . | Spleen . | |||||
|---|---|---|---|---|---|---|
| E . | G . | M . | GM . | GEMM . | Cells recovered (× 106) . | |
| PU.1+/+Spi-B+/+ | 4.7 ± 0.6 | 35.3 ± 10.5 | 25.7 ± 5.9 | 2.7 ± 0.7 | 11.1 ± 3.3 | 2.6 ± 0.9 |
| PU.1+/−Spi-B+/+ | 2.8 ± 0.8 | 24.3 ± 8.1 | 42.5 ± 18.6 | 4.3 ± 2.1 | 23.8 ± 13.2 | 2.7 ± 0.5 |
| PU.1+/−Spi-B+/− | 2.5 ± 0.6 | 37.8 ± 16.1 | 44.0 ± 12.8 | 6.5 ± 1.7 | 41.3 ± 7.9 | 2.1 ± 0.3 |
| PU.1+/+Spi-B−/− | 3.0 ± 1.2 | 22.5 ± 6.8 | 20.3 ± 7.5 | 3.8 ± 0.5 | 7.7 ± 2.6 | 2.6 ± 0.9 |
| PU.1+/−Spi-B−/− | 4.0 ± 0.6 | 45.8 ± 18.3 | 56.6 ± 11.6 | 6.0 ± 3.0 | 38.8 ± 9.6 | 2.1 ± 1.1 |
| Colony type . | Spleen . | |||||
|---|---|---|---|---|---|---|
| E . | G . | M . | GM . | GEMM . | Cells recovered (× 106) . | |
| PU.1+/+Spi-B+/+ | 4.7 ± 0.6 | 35.3 ± 10.5 | 25.7 ± 5.9 | 2.7 ± 0.7 | 11.1 ± 3.3 | 2.6 ± 0.9 |
| PU.1+/−Spi-B+/+ | 2.8 ± 0.8 | 24.3 ± 8.1 | 42.5 ± 18.6 | 4.3 ± 2.1 | 23.8 ± 13.2 | 2.7 ± 0.5 |
| PU.1+/−Spi-B+/− | 2.5 ± 0.6 | 37.8 ± 16.1 | 44.0 ± 12.8 | 6.5 ± 1.7 | 41.3 ± 7.9 | 2.1 ± 0.3 |
| PU.1+/+Spi-B−/− | 3.0 ± 1.2 | 22.5 ± 6.8 | 20.3 ± 7.5 | 3.8 ± 0.5 | 7.7 ± 2.6 | 2.6 ± 0.9 |
| PU.1+/−Spi-B−/− | 4.0 ± 0.6 | 45.8 ± 18.3 | 56.6 ± 11.6 | 6.0 ± 3.0 | 38.8 ± 9.6 | 2.1 ± 1.1 |
All values are expressed as mean ± SE.
E indicates erythroid colonies; G, granulocyte colonies; M, monocyte colonies; GM, mixed granulocyte and monocyte colonies; GEMM, multilineage colonies containing myeloid and erythroid cells.