Table 2. Effect of the MEK kinase... | American Society of Hematology

Table 2.

Effect of the MEK kinase inhibitor PD98059 on cytokine-mediated priming of arachidonate release in neutrophils

Stimulus, 1 μM	PD98059, μM	Priming agent, % control
Stimulus, 1 μM	PD98059, μM	FCS	TNF-α	GM-CSF
FMLP	0	—	100+	100
	1	—	80 ± 3^*	88 ± 10
	5	—	61 ± 3^*	49 ± 25
	10	—	52 ± 8^†	50 ± 3^*
	15	—	53 ± 24	46 ± 8^*
	20	—	28 ± 6^*	40 ± 8^†
	30	—	30 ± 4^*	57 ± 4^*
A23187	0	100	100	100
	1	79 ± 19	82 ± 8	79 ± 11
	5	71 ± 22	66 ± 17	67 ± 14
	10	80 ± 25	48 ± 5^†	59 ± 8^†
	15	73 ± 24	42 ± 8^†	46 ± 15^†
	20	86 ± 18	44 ± 8^†	54 ± 8^†
	30	80 ± 21	38 ± 4^*	40 ± 7^*

Stimulus, 1 μM	PD98059, μM	Priming agent, % control
Stimulus, 1 μM	PD98059, μM	FCS	TNF-α	GM-CSF
FMLP	0	—	100+	100
	1	—	80 ± 3^*	88 ± 10
	5	—	61 ± 3^*	49 ± 25
	10	—	52 ± 8^†	50 ± 3^*
	15	—	53 ± 24	46 ± 8^*
	20	—	28 ± 6^*	40 ± 8^†
	30	—	30 ± 4^*	57 ± 4^*
A23187	0	100	100	100
	1	79 ± 19	82 ± 8	79 ± 11
	5	71 ± 22	66 ± 17	67 ± 14
	10	80 ± 25	48 ± 5^†	59 ± 8^†
	15	73 ± 24	42 ± 8^†	46 ± 15^†
	20	86 ± 18	44 ± 8^†	54 ± 8^†
	30	80 ± 21	38 ± 4^*	40 ± 7^*

Purified neutrophils were incubated with PD98059 for 10 minutes at 37°C before priming and stimulation with agonist and measurement of arachidonate release as described in the legend to Table 1. Basal release of arachidonate in unstimulated samples was subtracted from the stimulated value, and the data were expressed as a percentage of DMSO diluent control. Absolute values were expressed as cpm/10⁶cells for (1) FMLP stimulation: FCS, 123 ± 142; TNF, 2563 ± 950; and GM-CSF, 1130 ± 30; and (2) A23187 stimulation; FCS, 2472 ± 779; TNF, 5266 ± 1251; and GM-CSF, 10 050 ± 524. Release of arachidonate from unprimed FMLP-stimulated cells was not significantly different from background, therefore the percent of control values was not determined. The data shown are the mean ± SE of 4 experiments.

See Table 1 for abbreviations.

.01 > P > .001 indicates significant difference between samples with inhibitor.

†

.05 > P > .01 indicates significant difference between samples without inhibitor.

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