Kinetics of donor T-cell engraftment early after transplantation in GCV-treated mice
| Days post-BMT . | GCV . | Spleen . | Thymus . | |||
|---|---|---|---|---|---|---|
| Percent donor T cells . | Percent TK+CD3+ T cells . | Percent donor Thy1.1 . | Percent donor . | |||
| CD4 . | CD8 . | |||||
| 4 | No | 4 | 1 | NE | 4 | 4 |
| Yes | 3 | 1 | NE | 5 | 3 | |
| 9 | No | 97 | 77 | 10 | 57 | 76 |
| Yes | 91 | 14 | 78 | 7 | 6 | |
| Days post-BMT . | GCV . | Spleen . | Thymus . | |||
|---|---|---|---|---|---|---|
| Percent donor T cells . | Percent TK+CD3+ T cells . | Percent donor Thy1.1 . | Percent donor . | |||
| CD4 . | CD8 . | |||||
| 4 | No | 4 | 1 | NE | 4 | 4 |
| Yes | 3 | 1 | NE | 5 | 3 | |
| 9 | No | 97 | 77 | 10 | 57 | 76 |
| Yes | 91 | 14 | 78 | 7 | 6 | |
Irradiated (850 cGy) AKR mice (Thy1.1+) were transplanted with TCD B6.PL (Thy1.1+) BM (107cells) plus 5 × 105 B6 (Thy 1.2+) TK+ T cells. Cohorts of mice were either treated with GCV (50 mg/kg) for 5 days beginning on day 4 after transplantation or left untreated. Representative mice from each group (N = 3/group) were killed on day 4 and day 9 after transplantation. Spleen and thymus cells from individual animals in each group were pooled and analyzed for chimerism. Donor Thy1.1+ cells in the spleen were defined as the percentage of donor Thy 1 cells that were Thy1.1+ and H-2b+. Donor CD4+ and CD8+ T cells in the thymus were defined as Thy1.2+CD4+ and Thy1.2+CD8+, respectively.
NE indicates not evaluable due to insufficient donor T-cell engraftment at day 4.