mRNA differently regulated in monocytes and neutrophils
| Gene symbol . | Gene name . | Neutrophil (control) . | Neutrophil +E coli . | Monocyte (control) . | Monocyte + E coli . |
|---|---|---|---|---|---|
| ANXA5 | Annexin A5 | 0 | 0 | 0 | 2 |
| SCYA7 | Small inducible cytokine A7 (monocyte chemotactic protein 3) | 0 | 0 | 0 | 3 |
| IL10RA | IL-10 receptor, alpha | 0 | 0 | 2 | 3 |
| HLA-DRA | MHC class II, DR alpha | 0 | 0 | 6 | 6 |
| CDW52 | CDW52 antigen (CAMPATH-1 antigen) | 0 | 0 | 5 | 4 |
| VIL2 | Villin 2 (ezrin) | 1 | 0 | 6 | 4 |
| HM74 | Putative chemokine receptor; GTP-binding protein | 1 | 6 | 0 | 0 |
| CD97 | CD97 antigen | 5 | 4 | 1 | 2 |
| PPIF | Peptidyl-prolyl isomerase F (cyclophilin F) | 3 | 8 | 5 | 3 |
| Gene symbol . | Gene name . | Neutrophil (control) . | Neutrophil +E coli . | Monocyte (control) . | Monocyte + E coli . |
|---|---|---|---|---|---|
| ANXA5 | Annexin A5 | 0 | 0 | 0 | 2 |
| SCYA7 | Small inducible cytokine A7 (monocyte chemotactic protein 3) | 0 | 0 | 0 | 3 |
| IL10RA | IL-10 receptor, alpha | 0 | 0 | 2 | 3 |
| HLA-DRA | MHC class II, DR alpha | 0 | 0 | 6 | 6 |
| CDW52 | CDW52 antigen (CAMPATH-1 antigen) | 0 | 0 | 5 | 4 |
| VIL2 | Villin 2 (ezrin) | 1 | 0 | 6 | 4 |
| HM74 | Putative chemokine receptor; GTP-binding protein | 1 | 6 | 0 | 0 |
| CD97 | CD97 antigen | 5 | 4 | 1 | 2 |
| PPIF | Peptidyl-prolyl isomerase F (cyclophilin F) | 3 | 8 | 5 | 3 |
Gene symbols are taken from the HGMW-approved symbol and/or LocusLink database of NCBI.65 Cells with E coli were incubated with opsonized E coli K12 for 2 hours as described in “Materials and methods.” The intensity of each band in the sequencing gels was quantified and expressed as a single-digit numeric as described in “Materials and methods.”