Flow cytometric analysis of K562/D+pBn, K562/D+B3, K562/D+B3SAO, and K562/D+B3EQ clones
| Clone . | FL1 for mouse negative control . | FL1 for BRIC 71 anti–band 3 . | FL1 for BRIC 69 anti-Rh30 . | FL1 for LA18.18 anti-RhAG . | FL1 for human negative control . | FL1 for Brad 5 anti-RhD . |
|---|---|---|---|---|---|---|
| K562/D+pBn clone 1 | 3.5 | 4.7 | 63 | 66 | 3.2 | 25 |
| K562/D+pBn clone 2 | 3.1 | 4.0 | 60 | 82 | 2.9 | 22 |
| K562/D+pBn clone 3 | 3.5 | 4.6 | 55 | 77 | 3.0 | 23 |
| K562/D+B3 clone 1 | 5.7 | 299 | 178 | 244 | 5.5 | 64 |
| K562/D+B3 clone 2 | 3.9 | 46 | 113 | 202 | 3.9 | 29 |
| K562/D+B3 clone 3 | 6.8 | 39 | 97 | 187 | 6.8 | 25 |
| K562/D+B3 clone 4 | 5.7 | 19 | 33 | 71 | 5.4 | 19 |
| K562/D+B3SAO clone 1 | 4.2 | 30 | 71 | 142 | 4.1 | 26 |
| K562/D+B3SAO clone 2 | 4.0 | 28 | 72 | 112 | 3.7 | 19 |
| K562/D+B3SAO clone 3 | 3.5 | 19 | 63 | 122 | 3.4 | 15 |
| K562/D+B3SAO clone 4 | 3.4 | 14 | 61 | 116 | 3.3 | 25 |
| K562/D+B3EQ clone 1 | 5.0 | 207 | 207 | 208 | 4.9 | 43 |
| K562/D+B3EQ clone 2 | 4.1 | 128 | 157 | 214 | 4.0 | 37 |
| K562/D+B3EQ clone 3 | 4.3 | 36 | 79 | 155 | 4.0 | 18 |
| K562/D+B3EQ clone 4 | 5.0 | 26 | 55 | 61 | 4.8 | 24 |
| Clone . | FL1 for mouse negative control . | FL1 for BRIC 71 anti–band 3 . | FL1 for BRIC 69 anti-Rh30 . | FL1 for LA18.18 anti-RhAG . | FL1 for human negative control . | FL1 for Brad 5 anti-RhD . |
|---|---|---|---|---|---|---|
| K562/D+pBn clone 1 | 3.5 | 4.7 | 63 | 66 | 3.2 | 25 |
| K562/D+pBn clone 2 | 3.1 | 4.0 | 60 | 82 | 2.9 | 22 |
| K562/D+pBn clone 3 | 3.5 | 4.6 | 55 | 77 | 3.0 | 23 |
| K562/D+B3 clone 1 | 5.7 | 299 | 178 | 244 | 5.5 | 64 |
| K562/D+B3 clone 2 | 3.9 | 46 | 113 | 202 | 3.9 | 29 |
| K562/D+B3 clone 3 | 6.8 | 39 | 97 | 187 | 6.8 | 25 |
| K562/D+B3 clone 4 | 5.7 | 19 | 33 | 71 | 5.4 | 19 |
| K562/D+B3SAO clone 1 | 4.2 | 30 | 71 | 142 | 4.1 | 26 |
| K562/D+B3SAO clone 2 | 4.0 | 28 | 72 | 112 | 3.7 | 19 |
| K562/D+B3SAO clone 3 | 3.5 | 19 | 63 | 122 | 3.4 | 15 |
| K562/D+B3SAO clone 4 | 3.4 | 14 | 61 | 116 | 3.3 | 25 |
| K562/D+B3EQ clone 1 | 5.0 | 207 | 207 | 208 | 4.9 | 43 |
| K562/D+B3EQ clone 2 | 4.1 | 128 | 157 | 214 | 4.0 | 37 |
| K562/D+B3EQ clone 3 | 4.3 | 36 | 79 | 155 | 4.0 | 18 |
| K562/D+B3EQ clone 4 | 5.0 | 26 | 55 | 61 | 4.8 | 24 |
B3SAO indicates band 3 found in Southeast Asian ovalocytosis; clone 1, clone with the highest obtained level of band 3 expression; Rh30, Rh polypeptides; RhAG, Rh glycoprotein.