Cooperative effect between CXC chemokines and Regakine-1 in neutrophil chemotaxis
| CXC chemokine* . | Concentration (ng/mL) . | Migration of neutrophils† . | |
|---|---|---|---|
| Buffer . | Regakine-1 . | ||
| IL-8 | 5 | 16.1 ± 3.7 | 53.3 ± 14.7 |
| 15 | 30.8 ± 3.4 | 61.8 ± 7.1 | |
| GCP-2 | 15 | 4.1 ± 2.4 | 10.2 ± 4.7 |
| 50 | 18.1 ± 7.0 | 36.2 ± 2.7 | |
| CXC chemokine* . | Concentration (ng/mL) . | Migration of neutrophils† . | |
|---|---|---|---|
| Buffer . | Regakine-1 . | ||
| IL-8 | 5 | 16.1 ± 3.7 | 53.3 ± 14.7 |
| 15 | 30.8 ± 3.4 | 61.8 ± 7.1 | |
| GCP-2 | 15 | 4.1 ± 2.4 | 10.2 ± 4.7 |
| 50 | 18.1 ± 7.0 | 36.2 ± 2.7 | |
IL-8 or GCP-2 was added as a chemoattractant to the lower wells of the microchamber.
Human neutrophils were added to the upper compartment of the microchamber after preincubation (10 minutes, 37°C) with buffer or 300 ng/mL synthetic Regakine-1. Results are expressed as the mean CI ± SEM of 3 independent experiments.