Oncostatin M promotes tissue factor antigen and activity
| Time (h) . | TF antigen (ng/mL) . | TF activity . | |
|---|---|---|---|
| Chromogenic assay (AU) . | Clotting assay (mU/mL) . | ||
| 0 | 0.41 ± 0.08 | 2.65 ± 0.80 | 2.35 ± 0.08 |
| 0.5 | 0.46 ± 0.05 | ND | 5.53 ± 0.35 |
| 1 | 1.54 ± 0.32 | 4.07 ± 1.25 | 5.27 ± 0.09 |
| 2 | 2.39 ± 0.48 | ND | 5.84 ± 0.05 |
| 4 | 2.37 ± 0.29 | 7.06 ± 2.21 | 5.99 ± 0.20 |
| 8 | 2.33 ± 0.23 | 3.95 ± 1.55 | 4.76 ± 0.07 |
| Time (h) . | TF antigen (ng/mL) . | TF activity . | |
|---|---|---|---|
| Chromogenic assay (AU) . | Clotting assay (mU/mL) . | ||
| 0 | 0.41 ± 0.08 | 2.65 ± 0.80 | 2.35 ± 0.08 |
| 0.5 | 0.46 ± 0.05 | ND | 5.53 ± 0.35 |
| 1 | 1.54 ± 0.32 | 4.07 ± 1.25 | 5.27 ± 0.09 |
| 2 | 2.39 ± 0.48 | ND | 5.84 ± 0.05 |
| 4 | 2.37 ± 0.29 | 7.06 ± 2.21 | 5.99 ± 0.20 |
| 8 | 2.33 ± 0.23 | 3.95 ± 1.55 | 4.76 ± 0.07 |
Kinetics of tissue factor antigen and activity induced by OSMs in smooth muscle cells. Cell cultures were incubated with OSM (10 ng/mL), and, at the indicated times, SMCs were lysed and TF antigen determined by ELISA. Lysates for TF activity were determined by chromogenic and clotting assays. Results are expressed as mean ± SD.
TF indicates tissue factor; AU, arbitrary unit; ND, not determined; OSM, oncostatin M; SMC, smooth muscle cell; ELISA, enzyme-linked immunosorbent assay.