Stem cell factor content of human peripheral blood eosinophils
| Patient . | Eos no./sample . | SCF (pg/mL) in fresh Eos . | SCF (pg/106) in fresh Eos . | SCF (pg/106) in overnight cultured Eos . | Purity of Eos (%) . |
|---|---|---|---|---|---|
| 1 | 3.0.106 | 22.7 | 7.6 | 22.3 | 98.0 |
| 2 | 3.0.106 | 19.3 | 6.4 | 8.8 | 98.7 |
| 3 | 6.0.106 | 51.8 | 8.6 | 7.2 | 99.6 |
| 4 | 2.0.106 | 46.2 | 23.1 | 9.2 | 99.2 |
| 5 | 8.4.106 | 63.2 | 7.5 | 10.3 | 100.0 |
| 6 | 6.5.106 | 44.5 | 6.8 | 10.4 | 99.4 |
| 7 | 2.4.106 | 31.1 | 12.9 | 7.3 | 99.7 |
| 8 | 2.2.106 | 14.4 | 6.5 | 11.0 | 98.0 |
| 9 | 4.5.106 | 19.8 | 4.4 | 4.8 | 98.7 |
| 10 | 5.8.106 | 27.2 | 4.7 | 4.9 | 98.6 |
| Mean ± SEM | 4.4.106 ± 0.7 | 34.0 ± 5.2 | 8.9 ± 1.7 | 9.6 ± 1.6 | 98.9 ± 0.2 |
| Patient . | Eos no./sample . | SCF (pg/mL) in fresh Eos . | SCF (pg/106) in fresh Eos . | SCF (pg/106) in overnight cultured Eos . | Purity of Eos (%) . |
|---|---|---|---|---|---|
| 1 | 3.0.106 | 22.7 | 7.6 | 22.3 | 98.0 |
| 2 | 3.0.106 | 19.3 | 6.4 | 8.8 | 98.7 |
| 3 | 6.0.106 | 51.8 | 8.6 | 7.2 | 99.6 |
| 4 | 2.0.106 | 46.2 | 23.1 | 9.2 | 99.2 |
| 5 | 8.4.106 | 63.2 | 7.5 | 10.3 | 100.0 |
| 6 | 6.5.106 | 44.5 | 6.8 | 10.4 | 99.4 |
| 7 | 2.4.106 | 31.1 | 12.9 | 7.3 | 99.7 |
| 8 | 2.2.106 | 14.4 | 6.5 | 11.0 | 98.0 |
| 9 | 4.5.106 | 19.8 | 4.4 | 4.8 | 98.7 |
| 10 | 5.8.106 | 27.2 | 4.7 | 4.9 | 98.6 |
| Mean ± SEM | 4.4.106 ± 0.7 | 34.0 ± 5.2 | 8.9 ± 1.7 | 9.6 ± 1.6 | 98.9 ± 0.2 |
Peripheral blood eosinophils from 10 different patients were sonicated immediately after isolation (fresh Eos) or after overnight culture in enriched medium, and SCF content was evaluated with the use of a commercial ELISA kit.