Oligonucleotides/probes used for semiquantitative polymerase chain reaction or Northern blot analysis
| Gene . | Primer sequence . | Length of PCR product . |
|---|---|---|
| CD87 | uni 5′GCTGCTCCACACCTGCGTCCCAGCCTC | 1005 bp |
| rev 5′CCAGCCAGGGCAGAGAGGGGGATTCT | ||
| PAI-2 | uni 5′AAACAATGGAGGATCTTTGTGTGG | 707 bp |
| rev 5′CATCTGTACAGGTGTGCGCTGAG | ||
| PAI-1 | uni 5′CTTGTCTTTGGTGAAGGGTCTGCTG | 887 bp |
| rev 5′CTTCCTGAGGTCGTCGACTTCAGTCTCC | ||
| MCP-1 | uni 5′CTGCTGCTCATAGCAGCCAC | 725 bp |
| rev 5′ACATCCCAGGGGTAGAACTG | ||
| Defensin | uni 5′GGCATTTATTTGAGATGAGG | 426 bp |
| rev 5′CCTCTCTGGTCACCCTGCCT | ||
| IP-10 | uni 5′CATGCAGAGCATATATCTATCTG | 656 bp |
| rev 5′CAAGGATGGACCACACAGAGGCTG | ||
| Adipophilin | uni 5′ATGATGCAGCTCGTGAGCAGT | 213 bp |
| rev 5′CTGCTGAGAGCCTGCTGGTAG | ||
| C3aR | uni 5′ACAACCATAATAGATGTGGC | 400 bp |
| rev 5′CGTAGAAGGAATTGCTAGAA | ||
| CD16 | uni 5′CAGCTGGCATGCGGACTGAAGATCTCC | 750 bp |
| rev 5′GGGTGACTTAAGAGATCTAGGGGTCGT | ||
| GAPDH | uni 5′GAAGGTGAAGGTCGGAGTCA | 505 bp |
| rev 5′GATACCAAAGTTGTCATGGA |
| Gene . | Primer sequence . | Length of PCR product . |
|---|---|---|
| CD87 | uni 5′GCTGCTCCACACCTGCGTCCCAGCCTC | 1005 bp |
| rev 5′CCAGCCAGGGCAGAGAGGGGGATTCT | ||
| PAI-2 | uni 5′AAACAATGGAGGATCTTTGTGTGG | 707 bp |
| rev 5′CATCTGTACAGGTGTGCGCTGAG | ||
| PAI-1 | uni 5′CTTGTCTTTGGTGAAGGGTCTGCTG | 887 bp |
| rev 5′CTTCCTGAGGTCGTCGACTTCAGTCTCC | ||
| MCP-1 | uni 5′CTGCTGCTCATAGCAGCCAC | 725 bp |
| rev 5′ACATCCCAGGGGTAGAACTG | ||
| Defensin | uni 5′GGCATTTATTTGAGATGAGG | 426 bp |
| rev 5′CCTCTCTGGTCACCCTGCCT | ||
| IP-10 | uni 5′CATGCAGAGCATATATCTATCTG | 656 bp |
| rev 5′CAAGGATGGACCACACAGAGGCTG | ||
| Adipophilin | uni 5′ATGATGCAGCTCGTGAGCAGT | 213 bp |
| rev 5′CTGCTGAGAGCCTGCTGGTAG | ||
| C3aR | uni 5′ACAACCATAATAGATGTGGC | 400 bp |
| rev 5′CGTAGAAGGAATTGCTAGAA | ||
| CD16 | uni 5′CAGCTGGCATGCGGACTGAAGATCTCC | 750 bp |
| rev 5′GGGTGACTTAAGAGATCTAGGGGTCGT | ||
| GAPDH | uni 5′GAAGGTGAAGGTCGGAGTCA | 505 bp |
| rev 5′GATACCAAAGTTGTCATGGA |
PCR indicates polymerase chain reaction; uni, forward primer; rev, reverse primer; bp, base pair.