Analysis of FVIIIa A2-subunit dissociation by optical biosensor
| . | koff(s−1) . | t1/2(In2/koff) . |
|---|---|---|
| pdFVIII | 4.9 (± 0.3) × 10−3 | 141 ± 6 s |
| FVIII WT | 5.9 (± 0.6) × 10−3 | 117 ± 9 s |
| ARG531HIS | 2.9 (± 0.2) × 10−2 | 24 ± 2 s |
| SER289LEU | 1.9 (± 0.1) × 10−2 | 36 ± 1.4 s |
| ALA284GLU | 2.0 (± 0.1) × 10−2 | 35 ± 1.6 s |
| BDD-FVIII | 4.2 (± 0.02) × 10−3 | 164 ± 2.6 s |
| BDD-SER289LEU | 2.3 (± 0.03) × 10−2 | 30 ± 1.2 s |
| . | koff(s−1) . | t1/2(In2/koff) . |
|---|---|---|
| pdFVIII | 4.9 (± 0.3) × 10−3 | 141 ± 6 s |
| FVIII WT | 5.9 (± 0.6) × 10−3 | 117 ± 9 s |
| ARG531HIS | 2.9 (± 0.2) × 10−2 | 24 ± 2 s |
| SER289LEU | 1.9 (± 0.1) × 10−2 | 36 ± 1.4 s |
| ALA284GLU | 2.0 (± 0.1) × 10−2 | 35 ± 1.6 s |
| BDD-FVIII | 4.2 (± 0.02) × 10−3 | 164 ± 2.6 s |
| BDD-SER289LEU | 2.3 (± 0.03) × 10−2 | 30 ± 1.2 s |