Table 2. Lack of repopulating activity... | American Society of Hematology

Table 2.

Lack of repopulating activity in the G₀ cells present in 5-day cultures of CD34⁺ cord blood cells

Experiment no.	G₁			G₀
	Cells per mouse^* (×10⁶)	Fraction of positive mice		Cells per mouse^*(×10⁶)	Fraction of positive mice
	Cells per mouse^* (×10⁶)	L⁺ + M⁺ (% human cells)^†	L⁺ ± M⁺	Cells per mouse^*(×10⁶)	L⁺ + M⁺	L⁺ ± M⁺
1	1.0	5/6 (2.1, 1.9, 3.4, 0.5, 4.4)	6/6	0.2	0/6	0/6
2	0.8	5/6 (11, 3, 12, 20, 9)	6/6	0.3	0/6	0/6
3	0.9	3/6 (0.6, 0.5, 1.5)	6/6	0.6	0/6	0/6

Experiment no.	G₁			G₀
	Cells per mouse^* (×10⁶)	Fraction of positive mice		Cells per mouse^*(×10⁶)	Fraction of positive mice
	Cells per mouse^* (×10⁶)	L⁺ + M⁺ (% human cells)^†	L⁺ ± M⁺	Cells per mouse^*(×10⁶)	L⁺ + M⁺	L⁺ ± M⁺
1	1.0	5/6 (2.1, 1.9, 3.4, 0.5, 4.4)	6/6	0.2	0/6	0/6
2	0.8	5/6 (11, 3, 12, 20, 9)	6/6	0.3	0/6	0/6
3	0.9	3/6 (0.6, 0.5, 1.5)	6/6	0.6	0/6	0/6

More than 90% of all cells in each fraction from the entire culture were injected into 6 irradiated NOD/SCID mice in each experiment.

†

% of human CD45/CD71⁺ cells in the bone marrow of those mice found to be positive 6 weeks after transplant (L⁺= at least 5 of 20 000 cells were human CD34⁻CD19/20⁺, M⁺ = at least 5 of 20 000 cells were human CD45/71⁺CD15/66b⁺cells).

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