Checkerboard analysis of BAECs in response to sPLA2
| sPLA2 in lower compartment (μmol/L) . | sPLA2 in upper compartment (μmol/L) . | ||
|---|---|---|---|
| 0 . | 1 . | 2 . | |
| 0 | 25 ± 2 | 28 ± 1 | 30 ± 4 |
| 1 | 57 ± 5 | 25 ± 2 | 26 ± 7 |
| 2 | 79 ± 3 | 58 ± 8 | 31 ± 6 |
| sPLA2 in lower compartment (μmol/L) . | sPLA2 in upper compartment (μmol/L) . | ||
|---|---|---|---|
| 0 . | 1 . | 2 . | |
| 0 | 25 ± 2 | 28 ± 1 | 30 ± 4 |
| 1 | 57 ± 5 | 25 ± 2 | 26 ± 7 |
| 2 | 79 ± 3 | 58 ± 8 | 31 ± 6 |
Various concentrations of bvPLA2 were placed in the upper and/or lower compartment as indicated. Cells were placed in the upper compartment of the Transwell chamber and allowed to migrate for 2 hours at 37°C and 5% CO2. Migration was quantified by counting the number of cells that migrated to the lower side of the polycarbonate membrane containing 8-μm pores. Numbers represent mean ± SE of 4 low-power fields (10 ×) from 3 wells.