Irradiated adult and conditioned newborn mice having transplantation with 10 T+ KSLA cells
| Experiment . | Recipients . | Positive mice . | % Chimerism . |
|---|---|---|---|
| 1 | Adult | 7/7 | 25.1 ± 19.5 (n = 7) |
| 2 | Adult | 6/6 | 41.9 ± 25.5 (n = 6) |
| Newborn | 8/8 | 17.2 ± 20.0 (n = 8) | |
| 3 | Newborn | 9/10 | 55.1 ± 22.2 (n = 9) |
| Experiment . | Recipients . | Positive mice . | % Chimerism . |
|---|---|---|---|
| 1 | Adult | 7/7 | 25.1 ± 19.5 (n = 7) |
| 2 | Adult | 6/6 | 41.9 ± 25.5 (n = 6) |
| Newborn | 8/8 | 17.2 ± 20.0 (n = 8) | |
| 3 | Newborn | 9/10 | 55.1 ± 22.2 (n = 9) |
Tie-2+, Kit+, Sca+, Lin−/low, AA4.1+ (T+ KSLA) cells were purified from fetal liver cells at day 14 of gestation. Ten purified cells were mixed with 2 × 105 adult bone marrow cells and transplanted to either irradiated adult or conditioned newborn mice. Peripheral blood cells of the recipient mice were analyzed for the presence of fetal liver–derived cells at 12 weeks after transplantation. Positive mice showed more than 1% chimerism. Chimerism (%) is presented as mean ± SD.