Tetanus toxoid–specific T-cell proliferation induced by immature (CD83−) or mature (CD83+) dendritic cells
| . | DC:T-cell ratio . | Experiment 1 . | Experiment 2 . | ||
|---|---|---|---|---|---|
| TT-SL . | Empty SL . | TT-SL . | Empty SL . | ||
| CD83+ | 1:10 | 51 751 | 17 933 | 27 522 | 5752 |
| 1:30 | ND | ND | 19 457 | 3116 | |
| CD83− | 1:10 | 41 121 | 23 879 | 13 893 | 1783 |
| 1:30 | ND | ND | 16 734 | 1642 | |
| . | DC:T-cell ratio . | Experiment 1 . | Experiment 2 . | ||
|---|---|---|---|---|---|
| TT-SL . | Empty SL . | TT-SL . | Empty SL . | ||
| CD83+ | 1:10 | 51 751 | 17 933 | 27 522 | 5752 |
| 1:30 | ND | ND | 19 457 | 3116 | |
| CD83− | 1:10 | 41 121 | 23 879 | 13 893 | 1783 |
| 1:30 | ND | ND | 16 734 | 1642 | |
Immature DC were generated and treated with MCM for 18 h. Cells were stained with anti-CD83 and FITC-conjugated goat-antimouse immunoglobulin and were sorted into CD83+ and CD83− fractions. DC were immediately transferred to 96-well flat-bottom trays, and 1 × 105 syngeneic T cells and TT-containing SL or empty SL were added. Mean cpm × 10−3 of triplicate cultures is shown.
ND, not determined.