Lineage distribution of αGal-expressing cells in reconstituted mice
| . | Spleen . | Lymph node . | Bone marrow . | Thymus . | Blood . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| % Lin +* . | % GT +† . | % Lin + . | % GT + . | % Lin + . | % GT + . | % Lin + . | % GT + . | % Lin + . | % GT + . | |
| Total | N/A | 48 ± 10 | N/A | 6 ± 2 | N/A | 44 ± 13 | N/A | 10 ± 4 | N/A | 12 ± 4 |
| B220 | 19 ± 2 | 61 ± 4 | 15 ± 4 | 18 ± 3 | 18 ± 4 | 50 ± 7 | N/A | N/A | 13 ± 3 | 52 ± 14 |
| F4/80 | 80 ± 5 | 4 ± 2 | 7 ± 4 | 3 ± 2 | 33 ± 4 | 47 ± 6 | 4 ± 3 | 22 ± 9 | 66 ± 10 | 10 ± 8 |
| CD3 | 13 ± 2 | 23 ± 3 | 7 ± 1 | 87 ± 2 | 12 ± 4 | 18 ± 6 | 5 ± 2 | 57 ± 10 | 8 ± 2 | 46 ± 15 |
| NK1.1 | 80 ± 8 | 4 ± 1 | 11 ± 2 | 6 ± 2 | 31 ± 3 | 35 ± 3 | 4 ± 1 | 58 ± 14 | 36 ± 9 | 11 ± 3 |
| . | Spleen . | Lymph node . | Bone marrow . | Thymus . | Blood . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| % Lin +* . | % GT +† . | % Lin + . | % GT + . | % Lin + . | % GT + . | % Lin + . | % GT + . | % Lin + . | % GT + . | |
| Total | N/A | 48 ± 10 | N/A | 6 ± 2 | N/A | 44 ± 13 | N/A | 10 ± 4 | N/A | 12 ± 4 |
| B220 | 19 ± 2 | 61 ± 4 | 15 ± 4 | 18 ± 3 | 18 ± 4 | 50 ± 7 | N/A | N/A | 13 ± 3 | 52 ± 14 |
| F4/80 | 80 ± 5 | 4 ± 2 | 7 ± 4 | 3 ± 2 | 33 ± 4 | 47 ± 6 | 4 ± 3 | 22 ± 9 | 66 ± 10 | 10 ± 8 |
| CD3 | 13 ± 2 | 23 ± 3 | 7 ± 1 | 87 ± 2 | 12 ± 4 | 18 ± 6 | 5 ± 2 | 57 ± 10 | 8 ± 2 | 46 ± 15 |
| NK1.1 | 80 ± 8 | 4 ± 1 | 11 ± 2 | 6 ± 2 | 31 ± 3 | 35 ± 3 | 4 ± 1 | 58 ± 14 | 36 ± 9 | 11 ± 3 |
N/A indicates not analyzed. Percentages shown represent the mean and standard deviation of quadruplicate samples from 3 mice analyzed at 18 weeks after bone marrow transplantation.
Percentage of lineage (Lin) marker positive cells that express αGa epitopes.
Percentage of αGal+ cells expressing each lineage marker.