Table 4. Increased blood absolute T-cell... | American Society of Hematology

Table 4.

Increased blood absolute T-cell counts following treatment

Patient		Absolute T-cell count (/μL)^4-150		% Increase	Week
Patient		Day 0	Peak	% Increase	Week
003	CD3⁺	1780	5440	206	1
	CD4⁺	680	1250	84
	CD8⁺	770	3810	395
004	CD3⁺	220	1280	482	3
	CD4⁺	60	600	900
	CD8⁺	140	560	300
005	CD3⁺	160	530	231	4
	CD4⁺	40	200	400
	CD8⁺	120	240	100
006	CD3⁺	910	4420	386	1
	CD4⁺	300	590	97
	CD8⁺	520	3410	556
007	CD3⁺	2240	4680	109	3
	CD4⁺	1120	3340	198
	CD8⁺	1000	1220	22
008	CD3⁺	3710	4990	35	1
	CD4⁺	460	2290	398
	CD8⁺	930	2040	119
009	CD3⁺	1080	5530	412	1
	CD4⁺	300	550	83
	CD8⁺	350	4430	1166
010	CD3⁺	1740	5500	216	4
	CD4⁺	520	1670	221
	CD8⁺	1040	3110	199
011	CD3⁺	2850	5610	97	2
	CD4⁺	1580	2010	27
	CD8⁺	1580	3010	91

Patient		Absolute T-cell count (/μL)^4-150		% Increase	Week
Patient		Day 0	Peak	% Increase	Week
003	CD3⁺	1780	5440	206	1
	CD4⁺	680	1250	84
	CD8⁺	770	3810	395
004	CD3⁺	220	1280	482	3
	CD4⁺	60	600	900
	CD8⁺	140	560	300
005	CD3⁺	160	530	231	4
	CD4⁺	40	200	400
	CD8⁺	120	240	100
006	CD3⁺	910	4420	386	1
	CD4⁺	300	590	97
	CD8⁺	520	3410	556
007	CD3⁺	2240	4680	109	3
	CD4⁺	1120	3340	198
	CD8⁺	1000	1220	22
008	CD3⁺	3710	4990	35	1
	CD4⁺	460	2290	398
	CD8⁺	930	2040	119
009	CD3⁺	1080	5530	412	1
	CD4⁺	300	550	83
	CD8⁺	350	4430	1166
010	CD3⁺	1740	5500	216	4
	CD4⁺	520	1670	221
	CD8⁺	1040	3110	199
011	CD3⁺	2850	5610	97	2
	CD4⁺	1580	2010	27
	CD8⁺	1580	3010	91

F4-150

Blood was collected from patients preinfusion (day 0), 1, 2, 3, 4, 8, and 12 weeks following treatment with Ad-CD154-CLL cells. A complete blood count with manual differential was performed and mononuclear cells were isolated by Ficoll-Hypaque density centrifugation. Mononuclear cells were stained with fluorochrome-labeled mouse antihuman CD3, CD4, and CD8 monoclonal antibody and isotype control then analyzed by flow cytometry to obtain percent CD3⁺, CD4⁺, and CD8⁺ cells. Absolute T-cell counts were calculated by multiplying absolute mononuclear cell count times percent CD3⁺, CD4⁺, or CD8⁺ cells. The peak time and absolute T-cell count are depicted.

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