Cytokine responses following treatment
| Patient . | Peak plasma cytokine level (h)3-150 . | ||
|---|---|---|---|
| IL-12 pg/mL . | IFN-γ ng/mL . | IL-6 pg/mL . | |
| 003 | 37 (8) | 2100 (24) | 115 (24) |
| 004 | < 10 (all) | 60 (24) | < 40 (all) |
| 005 | < 10 (all) | <4 (all) | < 40 (all) |
| 006 | 13 (4) | 271 (24) | 81 (24) |
| 007 | 177 (4) | 690 (24) | 60 (24) |
| 008 | 109 (8) | 333 (24) | 121 (24) |
| 009 | 135 (24) | 6000 (48) | 2557 (8) |
| 010 | 34 (8) | 490 (24) | < 40 (all) |
| 011 | 230 (8) | 695 (24) | 864 (24) |
| Patient . | Peak plasma cytokine level (h)3-150 . | ||
|---|---|---|---|
| IL-12 pg/mL . | IFN-γ ng/mL . | IL-6 pg/mL . | |
| 003 | 37 (8) | 2100 (24) | 115 (24) |
| 004 | < 10 (all) | 60 (24) | < 40 (all) |
| 005 | < 10 (all) | <4 (all) | < 40 (all) |
| 006 | 13 (4) | 271 (24) | 81 (24) |
| 007 | 177 (4) | 690 (24) | 60 (24) |
| 008 | 109 (8) | 333 (24) | 121 (24) |
| 009 | 135 (24) | 6000 (48) | 2557 (8) |
| 010 | 34 (8) | 490 (24) | < 40 (all) |
| 011 | 230 (8) | 695 (24) | 864 (24) |
Patient plasma was evaluated for IL-12, IFN-γ, and IL-6 by sandwich ELISA at 2, 4, 8, 24, 48, 72, and 168 hours following infusion of autologous Ad-CD154-CLL cells. Preinfusion (0 h) baseline levels of all cytokines were below the level of detection. Peak cytokine concentrations are expressed as the mean of triplicate determinations and estimated from a standard curve.