Table 2.

Bystander CD19+ leukemia B cells increase expression of CD95 following treatment

PatientMean fluorescence intensity ratio*
PreinfusionPostinfusion
003 1.5 3.8 
004 1.3 3.5  
005 1.5 1.9  
006 1.0 1.6 
007 1.0 2.8  
008 1.1 2.3  
009 1.1 2.6 
010 1.1 1.7  
011 1.3 1.9 
PatientMean fluorescence intensity ratio*
PreinfusionPostinfusion
003 1.5 3.8 
004 1.3 3.5  
005 1.5 1.9  
006 1.0 1.6 
007 1.0 2.8  
008 1.1 2.3  
009 1.1 2.6 
010 1.1 1.7  
011 1.3 1.9 
*

Patient blood was obtained prior to (preinfusion) and on the second day following (postinfusion) treatment with autologous Ad-CD154-CLL cells. Mononuclear cells were isolated by Ficoll-Hypaque density centrifugation then stained with fluorochrome-labeled mAb against CD19 and CD95 and analyzed by multicolor flow cytometry. Mean fluorescence intensity ratio is the ratio of mean fluorescence intensity of CD19+ cells stained with anti-CD95 mAb divided by mean fluorescence intensity of CD19+ cells incubated with isotype control mAb of irrelevant specificity.

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