Bystander CD19+ leukemia B cells increase expression of CD95 following treatment
Patient . | Mean fluorescence intensity ratio* . | |
---|---|---|
Preinfusion . | Postinfusion . | |
003 | 1.5 | 3.8 |
004 | 1.3 | 3.5 |
005 | 1.5 | 1.9 |
006 | 1.0 | 1.6 |
007 | 1.0 | 2.8 |
008 | 1.1 | 2.3 |
009 | 1.1 | 2.6 |
010 | 1.1 | 1.7 |
011 | 1.3 | 1.9 |
Patient . | Mean fluorescence intensity ratio* . | |
---|---|---|
Preinfusion . | Postinfusion . | |
003 | 1.5 | 3.8 |
004 | 1.3 | 3.5 |
005 | 1.5 | 1.9 |
006 | 1.0 | 1.6 |
007 | 1.0 | 2.8 |
008 | 1.1 | 2.3 |
009 | 1.1 | 2.6 |
010 | 1.1 | 1.7 |
011 | 1.3 | 1.9 |
Patient blood was obtained prior to (preinfusion) and on the second day following (postinfusion) treatment with autologous Ad-CD154-CLL cells. Mononuclear cells were isolated by Ficoll-Hypaque density centrifugation then stained with fluorochrome-labeled mAb against CD19 and CD95 and analyzed by multicolor flow cytometry. Mean fluorescence intensity ratio is the ratio of mean fluorescence intensity of CD19+ cells stained with anti-CD95 mAb divided by mean fluorescence intensity of CD19+ cells incubated with isotype control mAb of irrelevant specificity.