Percentage of expected human c-fes expression in various transgenic mouse tissues
| Construct . | Animal . | Copy no. . | Bone marrow . | Spleen . | Brain . | Lung . | Thymus . |
|---|---|---|---|---|---|---|---|
| 13.2 kb | 3 | 88 | 92.6 | 147.7 | 472.4 | 163.9 | 257.7 |
| Δ6-18b | 9 | 25 | 30.5 | 35 | 47.7 | 106.5 | 52.7 |
| 12 | 28 | 114.6 | 53.1 | 61.1 | 62.2 | 60.2 | |
| 13 | 7 | 106.2 | 141.5 | 240.2 | 165.5 | 138.7 | |
| 0.5-kb HSabc | 1 | 2 | 8.1 | 264.1 | 366.9 | 85 | 25.9 |
| 4 | 7 | 37.3 | 55.5 | 137 | 76.2 | 28.8 | |
| 7 | 40 | 15.8 | 25.2 | 89.3 | 44 | 250.6 | |
| 8 | 13 | — | 66.9 | 271.8 | 65 | 32 | |
| 10 | 5 | — | 13.3 | 313 | 19.8 | 21.6 | |
| 12 | 9 | 51.4 | 100 | 106.7 | 62 | — | |
| 21 | 1 | 56.2 | 96.6 | 198.8 | 377 | — | |
| 23 | 1 | 9.9 | 42.7 | 798.2 | 88.9 | 130.1 | |
| 25 | 2 | 11.4 | 12.4 | 156.6 | 12.9 | — |
| Construct . | Animal . | Copy no. . | Bone marrow . | Spleen . | Brain . | Lung . | Thymus . |
|---|---|---|---|---|---|---|---|
| 13.2 kb | 3 | 88 | 92.6 | 147.7 | 472.4 | 163.9 | 257.7 |
| Δ6-18b | 9 | 25 | 30.5 | 35 | 47.7 | 106.5 | 52.7 |
| 12 | 28 | 114.6 | 53.1 | 61.1 | 62.2 | 60.2 | |
| 13 | 7 | 106.2 | 141.5 | 240.2 | 165.5 | 138.7 | |
| 0.5-kb HSabc | 1 | 2 | 8.1 | 264.1 | 366.9 | 85 | 25.9 |
| 4 | 7 | 37.3 | 55.5 | 137 | 76.2 | 28.8 | |
| 7 | 40 | 15.8 | 25.2 | 89.3 | 44 | 250.6 | |
| 8 | 13 | — | 66.9 | 271.8 | 65 | 32 | |
| 10 | 5 | — | 13.3 | 313 | 19.8 | 21.6 | |
| 12 | 9 | 51.4 | 100 | 106.7 | 62 | — | |
| 21 | 1 | 56.2 | 96.6 | 198.8 | 377 | — | |
| 23 | 1 | 9.9 | 42.7 | 798.2 | 88.9 | 130.1 | |
| 25 | 2 | 11.4 | 12.4 | 156.6 | 12.9 | — |
Transgene copy number was quantified by Southern blot hybridization performed in triplicate. Murine c-fes RNA levels were given the arbitrary value 2, corresponding to the expression of both endogenous genes. Transgenic human c-fesRNA expression was compared with murine c-fes RNA levels and corrected for differences in gene copy number. For example, transgenic no. 3 (88 copies) would be expected to express 44 times as much humanc-fes RNA as murine c-fes RNA and listed as 100% of the expected value (2 copies). Thus, the value of humanc-fes expression is the volume of the human 273–base pair (bp) protected fragment (minus background) divided by the volume of the murine 288-bp protected fragment (minus background) and compared with the expected abundance on the basis of the copy number of each transgene. The value of human c-fes expression for all splenic samples is based on a single RPA autoradiogram for direct comparison.
HS indicates hypersensitive site; — indicates data were not determined.