Origin of hematopoietic cells and CFU-S12 in ossicle marrow
| Ossicle . | Origin of marrow cells from renal ossicles . | ||||
|---|---|---|---|---|---|
| Hematopoietic cells . | CFU-S12population . | ||||
| Donor (Ly-5.1−) . | Host (Ly-5.1+) . | Donor (zfy-1−/neo+) . | Host (zfy-1+/neo−) . | Endogenous (zfy-1−/neo−/M-CSF+) . | |
| 77d-N2 | 3.5% | 96.5% | 19/28 (67.86%) | 9/28 (32.14%) | 0/28 |
| 133d-N1 | ND | ND | 14/15 (93.33%) | 1/15 (6.67%) | 0/15 |
| 136d-N1 | 30.4% | 69.6% | 61/74 (83.56%) | 12/74 (16.44%) | 1/74 |
| 281d-N2 | 16.4% | 83.4% | 4/27 (16%) | 21/27 (84%) | 2/27 |
| Ossicle . | Origin of marrow cells from renal ossicles . | ||||
|---|---|---|---|---|---|
| Hematopoietic cells . | CFU-S12population . | ||||
| Donor (Ly-5.1−) . | Host (Ly-5.1+) . | Donor (zfy-1−/neo+) . | Host (zfy-1+/neo−) . | Endogenous (zfy-1−/neo−/M-CSF+) . | |
| 77d-N2 | 3.5% | 96.5% | 19/28 (67.86%) | 9/28 (32.14%) | 0/28 |
| 133d-N1 | ND | ND | 14/15 (93.33%) | 1/15 (6.67%) | 0/15 |
| 136d-N1 | 30.4% | 69.6% | 61/74 (83.56%) | 12/74 (16.44%) | 1/74 |
| 281d-N2 | 16.4% | 83.4% | 4/27 (16%) | 21/27 (84%) | 2/27 |
The contribution of BM-donor–derived (Ly-5.1−) and ossicle-host–derived (Ly-5.1+) hematopoietic cells was quantified in the bulk of the ossicle marrow by flow cytometry. The origin of the CFU-S12 population was determined on individually excised colonies by DNA hybridization with aneor gene probe (1200-bp EcoRI/SalI fragment from pXT1 plasmid DNA)15 and a male probe from thezfy-1 gene (1400-bp EcoRI/SalI fragment from pY2 plasmid).16 A probe for the single copy M-CSFgene (1100-bp EcoRI/HindIII fragment of plasmid pSPmM1)17was used as a DNA load control. Data are presented as the ratio of colonies of the respective origin versus the total number of CFU-S12 analyzed per ossicle. The percentage of donor and host-derived colonies with respect to the total number of exogenous colonies is shown in brackets.
CFU-S indicates spleen colony-forming unit; ND, not determined.