NK-cell development from Lin−SP cells
| Condition . | CD7+Lin−SP . | CD34+Lin− SP . | ||||
|---|---|---|---|---|---|---|
| Fold-expansion (range) . | % CD56+ (range) . | % CD56+ CD94+ (range) . | Fold-expansion (range) . | % CD56+ (range) . | % CD56+ CD94+ (range) . | |
| A | 4.3 ± 5.8 | 74.9 ± 19 | 25.1 ± 6.5 | 14.8 ± 17 | 68.4 ± 12 | 45.6 ± 8.3 |
| (0.1-13) | (53-87)4-150 | (18-29)4-150 | (0.1-35) | (55-78)4-150 | (40-55)4-150 | |
| B | 7.6 ± 11 | 55.9 ± 34 | 4-151 | 47.3 ± 67 | 5.1 ± 7.9 | 4-151 |
| (1.6-25) | (8.2-86) | (3.5-145) | (0.3-17) | |||
| C | 9.3 ± 7.0 | 91.0 ± 10‡ | 5.2 ± 6.7 | 2.8 ± 0.9 | 9.5 ± 13‡ | 4.2 ± 7.7 |
| (1.6-17) | (76-99) | (0-15) | (1.5-3.4) | (1.4-29) | (0-16) | |
| D | 20 ± 28 | 85.8 ± 16‡ | 28.1 ± 23 | 35.9 ± 74 | 34 ± 41‡ | 14 ± 25 |
| (4.4-68)4-153 | (62-96.8) | (4-41) | (1.4-168)4-153 | (0.9-86)4-155 | (0.3-52) | |
| Condition . | CD7+Lin−SP . | CD34+Lin− SP . | ||||
|---|---|---|---|---|---|---|
| Fold-expansion (range) . | % CD56+ (range) . | % CD56+ CD94+ (range) . | Fold-expansion (range) . | % CD56+ (range) . | % CD56+ CD94+ (range) . | |
| A | 4.3 ± 5.8 | 74.9 ± 19 | 25.1 ± 6.5 | 14.8 ± 17 | 68.4 ± 12 | 45.6 ± 8.3 |
| (0.1-13) | (53-87)4-150 | (18-29)4-150 | (0.1-35) | (55-78)4-150 | (40-55)4-150 | |
| B | 7.6 ± 11 | 55.9 ± 34 | 4-151 | 47.3 ± 67 | 5.1 ± 7.9 | 4-151 |
| (1.6-25) | (8.2-86) | (3.5-145) | (0.3-17) | |||
| C | 9.3 ± 7.0 | 91.0 ± 10‡ | 5.2 ± 6.7 | 2.8 ± 0.9 | 9.5 ± 13‡ | 4.2 ± 7.7 |
| (1.6-17) | (76-99) | (0-15) | (1.5-3.4) | (1.4-29) | (0-16) | |
| D | 20 ± 28 | 85.8 ± 16‡ | 28.1 ± 23 | 35.9 ± 74 | 34 ± 41‡ | 14 ± 25 |
| (4.4-68)4-153 | (62-96.8) | (4-41) | (1.4-168)4-153 | (0.9-86)4-155 | (0.3-52) | |
One hundred to 2000 CD7+Lin− SP cells or CD34+Lin− SP cells were cultured on MS5 stroma under the culture conditions defined in Table 1. The expansions and percentages of CD56+ or CD56+ CD94+progeny of CD7+Lin− SP or CD34+Lin− SP cells were derived from the total CD45+ cells present in the immunophenotyping studies. The data are the mean and SD of 4 (conditions A, B, and C) or 6 (condition D) separate experiments.
SP indicates side population.
One of 4 cultures failed to generate CD56+ progeny.
Too few CD56+ cells were elicited for an accurate determination.
P ≤ .05 for differences between the values for CD7+Lin− SP and CD34+Lin− SP.
Data are derived from 4 of 6 cultures with expansion.
Two of 4 cultures with expansion failed to generate CD56+ progeny.