Culture conditions for NK-cell development
| Condition . | Media and cytokines . |
|---|---|
| A | MEMα |
| 5 weeks: KL, F3L, IL-2, IL-7, IL-15 | |
| 2 weeks: IL-2 | |
| B | HAMS F12 |
| 1 week: KL, F3L, IL-2, IL-3, IL-7 | |
| 5 weeks: KL, F3L, IL-2, IL-7 | |
| 2 weeks: IL-2 | |
| C | HAMS F12 |
| 6 weeks: KL, IL-15 | |
| 2 weeks: IL-2 | |
| D | HAMS F12 |
| 2 weeks: KL, F3L, IL-2, IL-7, IL-15 | |
| 4 weeks: KL, IL-15 | |
| 2 weeks: KL, IL-2, IL-15 |
| Condition . | Media and cytokines . |
|---|---|
| A | MEMα |
| 5 weeks: KL, F3L, IL-2, IL-7, IL-15 | |
| 2 weeks: IL-2 | |
| B | HAMS F12 |
| 1 week: KL, F3L, IL-2, IL-3, IL-7 | |
| 5 weeks: KL, F3L, IL-2, IL-7 | |
| 2 weeks: IL-2 | |
| C | HAMS F12 |
| 6 weeks: KL, IL-15 | |
| 2 weeks: IL-2 | |
| D | HAMS F12 |
| 2 weeks: KL, F3L, IL-2, IL-7, IL-15 | |
| 4 weeks: KL, IL-15 | |
| 2 weeks: KL, IL-2, IL-15 |
Kit ligand (KL), Flt3 ligand (F3L), interleukin (IL)-2, IL-7, and/or IL-15 were used to propagate CD+ and CD34+Lin− SP cells on irradiated (20 Gy) MS5 stroma. IL-2 was used at 1000 U/mL, and IL-3 was used at 5 ng/mL. All other cytokines were at 10 ng/mL except under condition B, where KL and IL-7 were both used at 20 ng/mL.