Sickle cell and normal platelets do not activate HUVEC and do not augment activation of HUVEC by mononuclear leukocytes
| Addition to HUVEC . | n . | % Control . | |
|---|---|---|---|
| E-selectin . | ICAM-1 . | ||
| Sickle platelets | 3 | 104.8* | 85.8* |
| Normal platelets | 3 | 96.4* | 86.4* |
| Sickle mononuclear leukocytes | 1 | 375.0 | 145.5 |
| Sickle mononuclear leukocytes + sickle platelets | 1 | 281.0 | 152.5 |
| Sickle mononuclear leukocytes + normal platelets | 1 | 306.0 | 145.5 |
| Normal mononuclear leukocytes | 1 | 216.7 | 119.2 |
| Normal mononuclear leukocytes + normal platelets | 1 | 183.3 | 111.0 |
| Normal mononuclear leukocytes + sickle platelets | 1 | 184.5 | 108.1 |
| Addition to HUVEC . | n . | % Control . | |
|---|---|---|---|
| E-selectin . | ICAM-1 . | ||
| Sickle platelets | 3 | 104.8* | 85.8* |
| Normal platelets | 3 | 96.4* | 86.4* |
| Sickle mononuclear leukocytes | 1 | 375.0 | 145.5 |
| Sickle mononuclear leukocytes + sickle platelets | 1 | 281.0 | 152.5 |
| Sickle mononuclear leukocytes + normal platelets | 1 | 306.0 | 145.5 |
| Normal mononuclear leukocytes | 1 | 216.7 | 119.2 |
| Normal mononuclear leukocytes + normal platelets | 1 | 183.3 | 111.0 |
| Normal mononuclear leukocytes + sickle platelets | 1 | 184.5 | 108.1 |
HUVEC were incubated 4 hours with purified platelets (100 platelets per HUVEC), mononuclear leukocytes (2 mononuclear leukocytes per HUVEC), or platelets and mononuclear leukocytes (100 platelets and 2 mononuclear leukocytes per HUVEC). Note that because mononuclear preparations contained approximately 50 platelets per mononuclear leukocyte, the actual number of platelets per HUVEC was approximately 200 in incubations containing platelets and mononuclear leukocytes. E-selectin and ICAM-1 expression were measured in HUVEC by enzyme immunoassay. Control HUVEC were incubated with media only and equal 100%.
Not significantly different from control HUVEC.