Effect of huCD40LT on apoptosis, proliferation, and viability of cells in primary MCL cultures after 4 days of culture
| UPN# . | Apoptosis*(%) . | Proliferation† (%) . | Viability‡(%) . | |||
|---|---|---|---|---|---|---|
| Medium . | huCD40LT . | Medium . | huCD40LT . | Medium . | huCD40LT . | |
| 1 | 4.2 | 3.5 | 3.5 | 2.7 | 5.2 | 8.0 |
| 2 | 14.7 | 20.4 | 6.6 | 10.7 | 10.0 | 53.0 |
| 3 | 42.4 | 26.6 | 11.9 | 22.5 | 3.8 | 17.5 |
| 4 | 34.7 | 37.2 | 10.6 | 17.5 | 6.6 | 17 |
| 5 | 17.9 | 25.5 | 5.2 | 5.6 | 39.9 | 44.1 |
| 6 | 17.5 | 18.7 | 1.9 | 2.1 | 7.8 | 19.2 |
| 7 | 7.9 | 6.2 | 4.9 | 14.5 | ND | ND |
| Median | 19.9 | 19.7 | 6.4 | 10.8 | 12.2 | 26.5 |
| P value | .80 | .08 | .03 | |||
| UPN# . | Apoptosis*(%) . | Proliferation† (%) . | Viability‡(%) . | |||
|---|---|---|---|---|---|---|
| Medium . | huCD40LT . | Medium . | huCD40LT . | Medium . | huCD40LT . | |
| 1 | 4.2 | 3.5 | 3.5 | 2.7 | 5.2 | 8.0 |
| 2 | 14.7 | 20.4 | 6.6 | 10.7 | 10.0 | 53.0 |
| 3 | 42.4 | 26.6 | 11.9 | 22.5 | 3.8 | 17.5 |
| 4 | 34.7 | 37.2 | 10.6 | 17.5 | 6.6 | 17 |
| 5 | 17.9 | 25.5 | 5.2 | 5.6 | 39.9 | 44.1 |
| 6 | 17.5 | 18.7 | 1.9 | 2.1 | 7.8 | 19.2 |
| 7 | 7.9 | 6.2 | 4.9 | 14.5 | ND | ND |
| Median | 19.9 | 19.7 | 6.4 | 10.8 | 12.2 | 26.5 |
| P value | .80 | .08 | .03 | |||
UPN# indicates unique patient number; huCD40LT, 400 ng/mL of soluble recombinant human CD40 ligand trimer; and ND, not done.
Fraction of apoptotic cells as estimated by propidium iodide staining of isolated nuclei and flow cytometry.
Fraction of cells in the S/G2/M phases of the cell cycle as estimated by propidium iodide staining of isolated nuclei and flow cytometry.
Percent viability as determined by propidium iodide exclusion and scatter profiles using flow cytometry.