Ganciclovir-induced toxicity on LTC-IC after long-term culture assays
| . | Week 1 . | Week 6 . | |
|---|---|---|---|
| Supernatant . | Supernatant . | Adherent layer . | |
| Control (−) | 304 ± 40 | 64 ± 6 | 217 ± 10 |
| Control (+) | 400 ± 50 | 68 ± 7 | 213 ± 40 |
| Chimeric (−) | 184 ± 20 | 71 ± 9 | 300 ± 10 |
| Chimeric (+) | 20 ± 2 | 9 ± 5 | 7 ± 2 |
| . | Week 1 . | Week 6 . | |
|---|---|---|---|
| Supernatant . | Supernatant . | Adherent layer . | |
| Control (−) | 304 ± 40 | 64 ± 6 | 217 ± 10 |
| Control (+) | 400 ± 50 | 68 ± 7 | 213 ± 40 |
| Chimeric (−) | 184 ± 20 | 71 ± 9 | 300 ± 10 |
| Chimeric (+) | 20 ± 2 | 9 ± 5 | 7 ± 2 |
The presence of LTC-IC was determined weekly by the quantification of GM-CFC–derived colonies as described in the text. Values shown represent GM-CFC colonies/105 cells plated. Each number (± SD) is the mean of 3 wells in 2 separate experiments, using 2 independent chimeric animals. (−) Cultures performed without ganciclovir. (+) Cultures performed in the presence of ganciclovir.