Analysis of multipotent, mixed, and committed progenitor-derived colonies in semisolid assays
| Drug . | BFU-E . | GM-CFC . | CFU-Mk . | CFU-mix . | GEMMk . |
|---|---|---|---|---|---|
| Control cells | |||||
| None | 18 ± 2 | 130 ± 4 | 8 ± 1 | 10 ± 1 | 3 ± 2 |
| G 418 (1 mg/mL) | 0 | 0 | 0 | 0 | 0 |
| Ganciclovir (10 μM) | 17 | 129 ± 2 | 10 ± 1 | 8 ± 1 | 3 ± 1 |
| Chimeric cells | |||||
| None | 17 ± 1 | 140 ± 2 | 8 ± 1 | 7 ± 1 | 6 ± 2 |
| G 418 (1 mg/mL) | 26 ± 1 | 180 ± 1 | 17 ± 1 | 7 ± 1 | 4 ± 1 |
| Ganciclovir (10 μM) | 0 | 8 ± 3 | 0 | 0 | 0 |
| Drug . | BFU-E . | GM-CFC . | CFU-Mk . | CFU-mix . | GEMMk . |
|---|---|---|---|---|---|
| Control cells | |||||
| None | 18 ± 2 | 130 ± 4 | 8 ± 1 | 10 ± 1 | 3 ± 2 |
| G 418 (1 mg/mL) | 0 | 0 | 0 | 0 | 0 |
| Ganciclovir (10 μM) | 17 | 129 ± 2 | 10 ± 1 | 8 ± 1 | 3 ± 1 |
| Chimeric cells | |||||
| None | 17 ± 1 | 140 ± 2 | 8 ± 1 | 7 ± 1 | 6 ± 2 |
| G 418 (1 mg/mL) | 26 ± 1 | 180 ± 1 | 17 ± 1 | 7 ± 1 | 4 ± 1 |
| Ganciclovir (10 μM) | 0 | 8 ± 3 | 0 | 0 | 0 |
Marrow cells (5 × 104 per well) were obtained by femoral aspiration. Each number (± SD) is the mean of 3 wells in 3 different experiments, using 2 independent chimeric animals. CFU-mix consisted of colonies with erythroid and megakaryocytic component and colonies with granulocytic, macrophagic, and megakaryocytic without any erythroid component. Multipotent CFU-GEMMk were composed of granulocytic, erythrocytic, macrophagic and megakaryocytic components.