Flow cytometric analysis of adhesion receptor expression on LS174T, COLO205, and HCT-8 colon adenocarcinoma cell lines
| Receptor . | Geometric mean fluorescence . | ||
|---|---|---|---|
| LS174T . | COLO205 . | HCT-8 . | |
| Control IgG | 5.3 ± 0.3 | 5.1 ± 0.3 | 4.2 ± 0.3 |
| PSGL-1 | 11.7 ± 2.9 | 7.3 ± 0.7 | 4.2 ± 0.3 |
| CD24 | 264 ± 8.8 | 300 ± 27 | 4.5 ± 0.2 |
| CD24 + PI-PLC | 5.9 ± 0.9 | 27.9 ± 6.5 | nd |
| sLex | 722 ± 136 | 1204 ± 386 | 7.8 ± 1.0 |
| sLex + Neuraminidase | 215 ± 90 | 32 ± 18 | nd |
| αIIbB3 | 5.7 ± 0.2 | 5.2 ± 0.8 | nd |
| GPIb | 5.7 ± 0.3 | 5.6 ± 0.5 | nd |
| αvB3 | 8.3 ± 0.9 | 9.0 ± 1.1 | 8.4 ± 0.5 |
| αv | 35.6 ± 3.3 | nd | nd |
| PECAM-1 | 4.9 ± 0.2 | nd | nd |
| GPIV | 9.5 ± 0.2 | nd | nd |
| L1 | 21.1 ± 1.8 | 18.5 ± 2.4 | nd |
| Receptor . | Geometric mean fluorescence . | ||
|---|---|---|---|
| LS174T . | COLO205 . | HCT-8 . | |
| Control IgG | 5.3 ± 0.3 | 5.1 ± 0.3 | 4.2 ± 0.3 |
| PSGL-1 | 11.7 ± 2.9 | 7.3 ± 0.7 | 4.2 ± 0.3 |
| CD24 | 264 ± 8.8 | 300 ± 27 | 4.5 ± 0.2 |
| CD24 + PI-PLC | 5.9 ± 0.9 | 27.9 ± 6.5 | nd |
| sLex | 722 ± 136 | 1204 ± 386 | 7.8 ± 1.0 |
| sLex + Neuraminidase | 215 ± 90 | 32 ± 18 | nd |
| αIIbB3 | 5.7 ± 0.2 | 5.2 ± 0.8 | nd |
| GPIb | 5.7 ± 0.3 | 5.6 ± 0.5 | nd |
| αvB3 | 8.3 ± 0.9 | 9.0 ± 1.1 | 8.4 ± 0.5 |
| αv | 35.6 ± 3.3 | nd | nd |
| PECAM-1 | 4.9 ± 0.2 | nd | nd |
| GPIV | 9.5 ± 0.2 | nd | nd |
| L1 | 21.1 ± 1.8 | 18.5 ± 2.4 | nd |
Values are geometric mean fluorescence intensities ± SEM of n = 3-7 experiments using different batches of cells each time. nd, not done.