Release of intracellular iron mediated by LPS-triggered Mφ during acute GVHD
| Treatment in vitro . | Specific release of59Fe and 51Cr from dual-labeled MDW4 target cells, % . | |||
|---|---|---|---|---|
| Normal . | Acute GVHD . | |||
| 59Fe . | 51Cr . | 59Fe . | 51Cr . | |
| Medium | −0.1 ± 0.1 | 0.1 ± 0.1 | −0.7 ± 1.9 | −0.4 ± 1.6 |
| LPS 2.5 ng/mL | −0.7 ± 2.1 | −1.6 ± 1.8 | 41.4 ± 5.3 | 10.9 ± 2.0 |
| LPS 2.5 ng/mL + IFN-γ | 38.0 ± 10.2 | 9.0 ± 3.1 | 41.5 ± 7.8 | 9.5 ± 2.1 |
| LPS 2.5 ng/mL + anti–IFN-γ | 3.1 ± 2.5 | −0.3 ± 0.6 | 34.3 ± 12.1 | 3.0 ± 4.8 |
| LPS 2.5 ng/mL + IFN-γ + anti–IFN-γ | 4.9 ± 3.3 | −2.0 ± 1.8 | 35.2 ± 8.8 | 12.0 ± 4.8 |
| Treatment in vitro . | Specific release of59Fe and 51Cr from dual-labeled MDW4 target cells, % . | |||
|---|---|---|---|---|
| Normal . | Acute GVHD . | |||
| 59Fe . | 51Cr . | 59Fe . | 51Cr . | |
| Medium | −0.1 ± 0.1 | 0.1 ± 0.1 | −0.7 ± 1.9 | −0.4 ± 1.6 |
| LPS 2.5 ng/mL | −0.7 ± 2.1 | −1.6 ± 1.8 | 41.4 ± 5.3 | 10.9 ± 2.0 |
| LPS 2.5 ng/mL + IFN-γ | 38.0 ± 10.2 | 9.0 ± 3.1 | 41.5 ± 7.8 | 9.5 ± 2.1 |
| LPS 2.5 ng/mL + anti–IFN-γ | 3.1 ± 2.5 | −0.3 ± 0.6 | 34.3 ± 12.1 | 3.0 ± 4.8 |
| LPS 2.5 ng/mL + IFN-γ + anti–IFN-γ | 4.9 ± 3.3 | −2.0 ± 1.8 | 35.2 ± 8.8 | 12.0 ± 4.8 |
Mφ-mediated percent specific release from dual-labeled MDW4 target cells was determined in an 18-hour assay. Acute GVHD animals received 60 × 106 B6 cells. Mφ were isolated on day 14. Results are the mean ± SEM of 3 experiments. In the absence of Mφ, the percent specific release of 59Fe from cells single-labeled with 59Fe and incubated for 48 hours with 500 U/mL recombinant TNF-α equaled 0.1% ± 1.5% and −0.6% ± 1.5% for incubation with 500 U/mL recombinant TNF-α plus 1 μg/mL actinomycin D.