Predicted protein translations of the altered dCK cDNA constructs
| cDNA sequence . | T7B6 dCK-PCR, bp . | Coding region, bp . | Frame shift . | Predicted amino acids . | Predicted molecular weight . | dCK activity [nmol per min × mg] . |
|---|---|---|---|---|---|---|
| Wild type | 823 | 780 | No | 260 | 30.5 | 129.1 |
| Deletion exon 5 | 707 | 597 | Yes | 199 | 22.8 | 0 |
| Deletion exon 3-4 | 481 | 438 | No | 146 | 17.0 | 0 |
| Deletion exon 3-6 | 275 | 231 | No | 77 | 8.5 | 0 |
| Deletion exon 2-6 | 158 | 120 | Yes | — | — | — |
| cDNA sequence . | T7B6 dCK-PCR, bp . | Coding region, bp . | Frame shift . | Predicted amino acids . | Predicted molecular weight . | dCK activity [nmol per min × mg] . |
|---|---|---|---|---|---|---|
| Wild type | 823 | 780 | No | 260 | 30.5 | 129.1 |
| Deletion exon 5 | 707 | 597 | Yes | 199 | 22.8 | 0 |
| Deletion exon 3-4 | 481 | 438 | No | 146 | 17.0 | 0 |
| Deletion exon 3-6 | 275 | 231 | No | 77 | 8.5 | 0 |
| Deletion exon 2-6 | 158 | 120 | Yes | — | — | — |
Corresponding dCK activities as determined by the phosphorylation of 3H-labeled 2-CdA on 2.5 ng of dCK protein. Wild-type dCK was generated from a dCK T7B6-PCR construct from PHA T cells generated from healthy donors. The activity represents the mean activity in nmol/min × mg on 2.5-ng in vitro–translated dCK proteins from 5 individuals.