Effect of STI 571 on apoptosis in HMC-1 cells
| Condition . | Early apoptosis (% of stained cells) . | Late apoptosis (% of stained cells) . |
|---|---|---|
| Medium only | 3.6 | 5.7 |
| 10 μmol/L STI 571 | 52.7 | 31.9 |
| 1 μmol/L STI 571 | 63.7 | 32.0 |
| 0.1 μmol/L STI 571 | 30.7 | 13.2 |
| 0.01 μmol/L STI 571 | 4.9 | 4.3 |
| Condition . | Early apoptosis (% of stained cells) . | Late apoptosis (% of stained cells) . |
|---|---|---|
| Medium only | 3.6 | 5.7 |
| 10 μmol/L STI 571 | 52.7 | 31.9 |
| 1 μmol/L STI 571 | 63.7 | 32.0 |
| 0.1 μmol/L STI 571 | 30.7 | 13.2 |
| 0.01 μmol/L STI 571 | 4.9 | 4.3 |
HMC-1 cells were grown in RPMI-1640 and 10% FCS with and without various concentrations of STI 571. Apoptosis at 72 hours was assessed by using a flow cytometric assay to measure cellular binding of an annexin V–FITC conjugate, as well as uptake of the vital stain 7-AAD. A total of 10 000 cells were analyzed for each condition. Results are expressed as the percentage of cells that stained positively for annexin V-FITC only (early apoptosis) or for both annexin V-FITC and 7-AAD (late apoptosis). Representative results from 1 of 4 independent experiments are shown.