Th1 but not Th2 lymphokine secretion is impaired by Lptn
| Stimulus . | IL-2 (day 1) . | IFN-γ (day 3) . | IL-4 (day 3) . |
|---|---|---|---|
| CD3 | 0.44 (0.04) | 7.03 (2.30) | 0.75 (0.45) |
| CD3 + Lptn | 0.09 (0.04) | 3.77 (1.99) | 0.75 (0.49) |
| CD3 + Lptn + anti-Lptn | 0.39 (0.09) | 7.65 (2.17) | 0.74 (0.44) |
| CD3 + RANTES | 0.44 (0.19) | 8.43 (0.81) | 0.67 (0.41) |
| CD3 + RANTES + anti-RANTES | 0.38 (0.08) | 7.43 (1.20) | 0.63 (0.36) |
| CD3 + CD28 | 2.76 (1.55) | 17.2 (7.85) | 0.45 (0.23) |
| Stimulus . | IL-2 (day 1) . | IFN-γ (day 3) . | IL-4 (day 3) . |
|---|---|---|---|
| CD3 | 0.44 (0.04) | 7.03 (2.30) | 0.75 (0.45) |
| CD3 + Lptn | 0.09 (0.04) | 3.77 (1.99) | 0.75 (0.49) |
| CD3 + Lptn + anti-Lptn | 0.39 (0.09) | 7.65 (2.17) | 0.74 (0.44) |
| CD3 + RANTES | 0.44 (0.19) | 8.43 (0.81) | 0.67 (0.41) |
| CD3 + RANTES + anti-RANTES | 0.38 (0.08) | 7.43 (1.20) | 0.63 (0.36) |
| CD3 + CD28 | 2.76 (1.55) | 17.2 (7.85) | 0.45 (0.23) |
The CD4+ T cells were activated with the indicated stimuli, as described in “Materials and methods.” Supernatants were collected each day from days 1 to 3 and were analyzed for lymphokine concentrations by ELISA. Concentration mean values (ng/mL) are followed by the SD in parentheses. In our hands, only stimulation with PMA and ionomycin gave 2 times higher IL-4 secreted levels than CD3 stimulation, alone or combined with CD28. For each lymphokine, data are representative of 4 independent experiments, except for conditions using RANTES, where 2 experiments were done.