Table 3. Incidence of somatically... | American Society of Hematology

Table 3.

Incidence of somatically mutated V_Htranscripts in lymphocyte cocultures is dependent primarily on the B-cell donor

Experiment	Source of B cells	Source of T cells	Number of transcripts		Mutated transcripts^3-150 (%)	Nucleotide substitutes^3-151 (%)
Experiment	Source of B cells	Source of T cells	V_H3	V3-23	Mutated transcripts^3-150 (%)	Nucleotide substitutes^3-151 (%)
Exp 1	BMT1	BMT1	nd³	161	12
	BMT1	HS1	nd	100	8
	HS1	BMT1	nd	138	13
	HS1	HS1	nd	155	57
Exp 2	BMT2	HS1	344	151	8
Exp 3	BMT3	BMT3	nd	53	15
	BMT3	HS3	374	99	1
	BMT4	HS3	>354	54	86
	HS3	BMT3	712	37	86
	HS3	HS3	>375	11	65
	BMT3	—	726	206	1
	BMT4	—	482	59	1
	HS3	—	381	20	10
Exp 4	BMT6	BMT6	461	74	1
	BMT5	BMT5	922	254	13	0.2
	BMT6	HS4	412	109	10
	BMT5	HS4	>742	98	1
	HS4	BMT5	620	273	64	3.1
	HS4	HS4	491	74	60
	BMT6	—	570	155	11
	BMT5	—	789	163	12
	HS4	—	86	12	0
Exp 5	BMT7	BMT7	647	152	7
	BMT7	HS5	751	212	6
	HS5	BMT7	601	171	14
	HS5	HS5	754	137	19
	BMT7	—	745	139	11
	HS5	—	820	168	7
Exp 6	BMT8	BMT8	353	90	28
	BMT8	HS6	664	87	21
	HS6	BMT8	681	90	88
	HS6	HS6	736	164	75
	BMT8	—	784	130	18
	HS6	—	683	153	19
Exp 7	BMT9	BMT9	345	58	33
	BMT9	HS4	331	70	23
	HS4	BMT9	288	142	20
	HS4	HS4	>1320	194	82
	BMT9	—	684	98	7
	HS4	—	697	176	33

Experiment	Source of B cells	Source of T cells	Number of transcripts		Mutated transcripts^3-150 (%)	Nucleotide substitutes^3-151 (%)
Experiment	Source of B cells	Source of T cells	V_H3	V3-23	Mutated transcripts^3-150 (%)	Nucleotide substitutes^3-151 (%)
Exp 1	BMT1	BMT1	nd³	161	12
	BMT1	HS1	nd	100	8
	HS1	BMT1	nd	138	13
	HS1	HS1	nd	155	57
Exp 2	BMT2	HS1	344	151	8
Exp 3	BMT3	BMT3	nd	53	15
	BMT3	HS3	374	99	1
	BMT4	HS3	>354	54	86
	HS3	BMT3	712	37	86
	HS3	HS3	>375	11	65
	BMT3	—	726	206	1
	BMT4	—	482	59	1
	HS3	—	381	20	10
Exp 4	BMT6	BMT6	461	74	1
	BMT5	BMT5	922	254	13	0.2
	BMT6	HS4	412	109	10
	BMT5	HS4	>742	98	1
	HS4	BMT5	620	273	64	3.1
	HS4	HS4	491	74	60
	BMT6	—	570	155	11
	BMT5	—	789	163	12
	HS4	—	86	12	0
Exp 5	BMT7	BMT7	647	152	7
	BMT7	HS5	751	212	6
	HS5	BMT7	601	171	14
	HS5	HS5	754	137	19
	BMT7	—	745	139	11
	HS5	—	820	168	7
Exp 6	BMT8	BMT8	353	90	28
	BMT8	HS6	664	87	21
	HS6	BMT8	681	90	88
	HS6	HS6	736	164	75
	BMT8	—	784	130	18
	HS6	—	683	153	19
Exp 7	BMT9	BMT9	345	58	33
	BMT9	HS4	331	70	23
	HS4	BMT9	288	142	20
	HS4	HS4	>1320	194	82
	BMT9	—	684	98	7
	HS4	—	697	176	33

Nd indicates not determined; BMT, bone marrow transplant; HS, healthy subject. Dash indicates that no T cells were added but that B-cell cultures were supported by L cells supplemented with CD40L (1 μg/mL).

F3-150

Percentage of V3-23 transcripts with mutation detectable by hybridization.

F3-151

Incidence (%) of nucleotide substitution per base pair (bp) based on 290 bp/transcript of sequence shown in Figure 4.

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