Dose response of LIF on the production of SCEPF by stromal cells
| LIF Doses in Stromal Cultures . | Frequency of CD34+ thy-1+/Positive Wells1-1-150 . | Percentage of CD34+ Thy-1+ Cells1-1-151 . |
|---|---|---|
| 10 ng | 100% (10/10) | 15 ± 3 |
| 5 ng | 100% (10/10) | 15 ± 4 |
| 2.5 ng | 100% (10/10) | 15 ± 5 |
| 1 ng | 100% (10/10) | 15 ± 4 |
| 500 pg | 100% (10/10) | 14 ± 5 |
| 250 pg | 60% (6/10) | 8 ± 3 |
| 100 pg | 20% (2/10) | 3.6 and 2.6 |
| 50 pg | 0% (0/10) | N/A |
| 25 pg | 0% (0/10) | N/A |
| 10 pg | 0% (0/10) | N/A |
| LIF Doses in Stromal Cultures . | Frequency of CD34+ thy-1+/Positive Wells1-1-150 . | Percentage of CD34+ Thy-1+ Cells1-1-151 . |
|---|---|---|
| 10 ng | 100% (10/10) | 15 ± 3 |
| 5 ng | 100% (10/10) | 15 ± 4 |
| 2.5 ng | 100% (10/10) | 15 ± 5 |
| 1 ng | 100% (10/10) | 15 ± 4 |
| 500 pg | 100% (10/10) | 14 ± 5 |
| 250 pg | 60% (6/10) | 8 ± 3 |
| 100 pg | 20% (2/10) | 3.6 and 2.6 |
| 50 pg | 0% (0/10) | N/A |
| 25 pg | 0% (0/10) | N/A |
| 10 pg | 0% (0/10) | N/A |
Stromal conditioned mediums (SCMs) were prepared from stromal cultures treated with various doses of LIF. Preparation of SCM and the assay for the SCEPF activity in the SCMs were performed as previously described.1-1 1-2
A well is scored as CD34+ thy-1+/positive only if it has detectable (>1%) of CD34+ thy-1+cells in the well. Numbers in parentheses indicate the total number of CD34+ thy-1+/positive wells divided by the total number of wells analyzed in the experiments.
Data is presented as the mean ± SD of the total number of CD34+ thy-1+/positive wells in each culture condition. N/A indicates not applicable.