Effect of deregulated E2F-1 on leukemogenicity of M1 cells
| Cells* . | IL-6 . | Mice dead (%) . | Myeloid leukemic cells recovered from bone marrow† . | |
|---|---|---|---|---|
| 3 wk . | 6 wk . | |||
| None | − | 0 | 0 | − |
| M1 | − | 60 | 100 | + |
| M1 | + | 0 | 0 | − |
| M1E2F-1 | − | 40 | 100 | + |
| M1E2F-1 | + | 50 | 100 | + |
| Cells* . | IL-6 . | Mice dead (%) . | Myeloid leukemic cells recovered from bone marrow† . | |
|---|---|---|---|---|
| 3 wk . | 6 wk . | |||
| None | − | 0 | 0 | − |
| M1 | − | 60 | 100 | + |
| M1 | + | 0 | 0 | − |
| M1E2F-1 | − | 40 | 100 | + |
| M1E2F-1 | + | 50 | 100 | + |
For each cell type, 10 nude mice were intravenously (tail) injected with 2 × 106 cells. Cells were treated for 5 days with or without IL-6 (50 ng/mL) prior to inoculation of the mice.
Three weeks following inoculation, one mouse injected with each cell type was sacrificed, and the bone marrow was analyzed for the presence of myeloid leukemic cells.