Trfhpx/hpx mice have a mutation disrupting the exon 16 splice donor site
| Allele . | Exon 16 donor . | Exon 17 acceptor . |
|---|---|---|
| Wild type | CCTCACgtaag | cccagGACTCC |
| hpx/mutation | CCTCACataag | cccagGACTCC |
| hpx/cryptic | CTGTCGgtaac | cccagGACTCC |
| Mammalian consensus | NNNCAGgtaag | cncagGNNNNN |
| Allele . | Exon 16 donor . | Exon 17 acceptor . |
|---|---|---|
| Wild type | CCTCACgtaag | cccagGACTCC |
| hpx/mutation | CCTCACataag | cccagGACTCC |
| hpx/cryptic | CTGTCGgtaac | cccagGACTCC |
| Mammalian consensus | NNNCAGgtaag | cncagGNNNNN |
Sequences are shown for coding (uppercase) and noncoding (lowercase) nucleotides surrounding the exon 16 splice donor site. Thehpx mutation is underlined. The alternative, cryptic splice site, found within exon 16 coding sequence is also indicated. The lower case, italicized nucleotides represent coding sequence that becomes intron sequence with the aberrant splice event. The mammalian consensus sequence was the best fit of several mammalian donor/acceptor consensus sequences described by Shapiro and Senapathy.26