Proliferative activity of T-ALL cells
| . | 0 h . | Medium . | +IL-2 . | +IL-4 . | +IL-7 . |
|---|---|---|---|---|---|
| % Cells in S, G2/M phases* | 10.3 ± 1.2 | 9.3 ± 1.2 | 8.7 ± 1.2 | 10.0 ± 1.2 | 9.9 ± 1.4 |
| Range (%) | 2-26 | 0-29 | 2-27 | 1-32 | 1-38 |
| No. patients | 36 | 38 | 34 | 38 | 38 |
| . | 0 h . | Medium . | +IL-2 . | +IL-4 . | +IL-7 . |
|---|---|---|---|---|---|
| % Cells in S, G2/M phases* | 10.3 ± 1.2 | 9.3 ± 1.2 | 8.7 ± 1.2 | 10.0 ± 1.2 | 9.9 ± 1.4 |
| Range (%) | 2-26 | 0-29 | 2-27 | 1-32 | 1-38 |
| No. patients | 36 | 38 | 34 | 38 | 38 |
Leukemic cells were analyzed for DNA content before and after incubation in the standard medium or with cytokines for 24 hours at 37°C. Cell cycle analysis was performed by flow cytometry on ethanol-fixed, RNase-treated, and PI-stained cells. Proliferative activity was quantified as percentage of cells in the S and G2/M phases.
Average values (mean ± SEM) are presented. Experiments were performed at least twice for each leukemic cell sample.