Differential effects of cytokines on spontaneous apoptosis in T-ALL
| Apoptosis-modulating effects* . | IL-2 (%) . | IL-4 (%) . | IL-7 (%) . |
|---|---|---|---|
| Inhibition | 0/37 (0)† | 7/37 (19) | 24/38 (63) |
| No effect | 36/37 (97) | 28/37 (76) | 14/38 (37) |
| Induction | 1/37 (3) | 2/37 (5) | 0/38 (0) |
| Apoptosis-modulating effects* . | IL-2 (%) . | IL-4 (%) . | IL-7 (%) . |
|---|---|---|---|
| Inhibition | 0/37 (0)† | 7/37 (19) | 24/38 (63) |
| No effect | 36/37 (97) | 28/37 (76) | 14/38 (37) |
| Induction | 1/37 (3) | 2/37 (5) | 0/38 (0) |
Cells were incubated in the presence and absence of cytokines for 24 hours at 37°C and were assessed for apoptosis by flow cytometry, and the cytokine-specific apoptosis modulation (ΔN) was calculated as the difference between apoptosis extents in the presence and absence of the corresponding cytokine. Experiments were performed at least twice for each leukemic cell sample, with a mean value of standard deviation (SD) of 2%. The 2.5-fold SD-value (ie, ΔNIL-7 = 5%) was used as a threshold value for the cytokine-induced modulation of apoptosis.
Number of patients/total number of patients (percentage of patients).