Effect of MoAb 6D1 on shear-induced platelet aggregation in the presence of type 2B rvWFs and rvWF-WT
| . | Disappearance of single platelets (%) . | |||
|---|---|---|---|---|
| 200 seconds−1 . | 4000 seconds−1 . | |||
| . | None . | MoAb 6D1 . | None . | MoAb 6D1 . |
| WT | 13.3 ± 5.9 | ND | 40.4 ± 1.6 | 6.4 ± 4.7 |
| L697V | 12.2 ± 5.6 | ND | 83.5 ± 3.8 | 3.5 ± 0.7 |
| R578Q | 62.5 ± 10.1 | 2.3 ± 3.2 | 91.7 ± 3.2 | 8.9 ± 3 |
| V551F | 59.1 ± 5.4 | 8.7 ± 1.6 | 89.5 ± 4.8 | 7.8 ± 6.5 |
| V553M | 76.6 ± 5.7 | 6.1 ± 2.5 | 95.9 ± 1.7 | 16.2 ± 3.8 |
| M540MM | 85.9 ± 4.3 | 9.9 ± 2.9 | 94.8 ± 1 | 10.2 ± 2.5 |
| . | Disappearance of single platelets (%) . | |||
|---|---|---|---|---|
| 200 seconds−1 . | 4000 seconds−1 . | |||
| . | None . | MoAb 6D1 . | None . | MoAb 6D1 . |
| WT | 13.3 ± 5.9 | ND | 40.4 ± 1.6 | 6.4 ± 4.7 |
| L697V | 12.2 ± 5.6 | ND | 83.5 ± 3.8 | 3.5 ± 0.7 |
| R578Q | 62.5 ± 10.1 | 2.3 ± 3.2 | 91.7 ± 3.2 | 8.9 ± 3 |
| V551F | 59.1 ± 5.4 | 8.7 ± 1.6 | 89.5 ± 4.8 | 7.8 ± 6.5 |
| V553M | 76.6 ± 5.7 | 6.1 ± 2.5 | 95.9 ± 1.7 | 16.2 ± 3.8 |
| M540MM | 85.9 ± 4.3 | 9.9 ± 2.9 | 94.8 ± 1 | 10.2 ± 2.5 |
Washed platelets (0.5 × 108/mL) were preincubated with MoAb 6D1 (20 μg/mL), rvWF (1 μg/mL) was added, and the mixture was subjected to a shear rate of 200 seconds−1 or 4000 seconds−1. The results were expressed as the percentage of disappearance of single platelets in the absence or in the presence of MoAb 6D1. Means ± SEM from 3 experiments were performed in duplicate.
MoAb 6D1 was able to inhibit SIPA induced either by type 2B rvWF or by rvWF-WT-containing samples relative to the sample in the absence of antibody, both at 200 and 4000 seconds−1. ND indicates not done.