Percentage of clonogenic progenitors within subpopulations of CD34−CD38−Lin− and CD34+CD38−Lin− cells
| Subpopulation Phenotype . | CFU-M, % . | CFU-G, % . | CFU-GM, % . | BFU-E, % . | CFU-GEMM, % . |
|---|---|---|---|---|---|
| 34−38−7− | 4.3 ± 1.7 | 25 ± 7.5 | 0.5 ± 0.5 | 65 ± 9.5 | 5.2 ± 0.9 |
| 34−38−7+ | 1.3 ± 1.3 | 25.5 ± 17.5 | 0 | 73 ± 16 | 0 |
| 34+38−7− | 12.3 ± 2.9 | 43.5 ± 10.4 | 3.3 ± 1.8 | 36.8 ± 9.5 | 4 ± 2 |
| 34+38−7+ | 20.1 ± 13.5 | 53.6 ± 15.6 | 0 | 20.4 ± 11.9 | 5.6 ± 3.2 |
| 34−38−AC133− | 1.4 ± 1.4 | 45.6 ± 37.4 | 0 | 52.1 ± 35.5 | 0.7 ± 0.7 |
| 34−38−AC133+ | 2.9 ± 1.5 | 36.2 ± 6.5 | 1.2 ± 1.2 | 54.3 ± 8.3 | 5.2 ± 3.6 |
| 34+38−AC133− | 2.6 ± 1.6 | 31.5 ± 5.3 | 0.4 ± 0.4 | 61.4 ± 8.3* | 3.8 ± 1.1 |
| 34+38−AC133+ | 11.7 ± 0.5 | 66 ± 5.7 | 2.5 ± 2.0 | 18.4 ± 3.0 | 1.7 ± 1.3 |
| Subpopulation Phenotype . | CFU-M, % . | CFU-G, % . | CFU-GM, % . | BFU-E, % . | CFU-GEMM, % . |
|---|---|---|---|---|---|
| 34−38−7− | 4.3 ± 1.7 | 25 ± 7.5 | 0.5 ± 0.5 | 65 ± 9.5 | 5.2 ± 0.9 |
| 34−38−7+ | 1.3 ± 1.3 | 25.5 ± 17.5 | 0 | 73 ± 16 | 0 |
| 34+38−7− | 12.3 ± 2.9 | 43.5 ± 10.4 | 3.3 ± 1.8 | 36.8 ± 9.5 | 4 ± 2 |
| 34+38−7+ | 20.1 ± 13.5 | 53.6 ± 15.6 | 0 | 20.4 ± 11.9 | 5.6 ± 3.2 |
| 34−38−AC133− | 1.4 ± 1.4 | 45.6 ± 37.4 | 0 | 52.1 ± 35.5 | 0.7 ± 0.7 |
| 34−38−AC133+ | 2.9 ± 1.5 | 36.2 ± 6.5 | 1.2 ± 1.2 | 54.3 ± 8.3 | 5.2 ± 3.6 |
| 34+38−AC133− | 2.6 ± 1.6 | 31.5 ± 5.3 | 0.4 ± 0.4 | 61.4 ± 8.3* | 3.8 ± 1.1 |
| 34+38−AC133+ | 11.7 ± 0.5 | 66 ± 5.7 | 2.5 ± 2.0 | 18.4 ± 3.0 | 1.7 ± 1.3 |
CFU-M indicates CFU-macrophage; CFU-G, CFU-granulocyte; CFU-GM, CFU-granulocyte, macrophage; BFU-E, burst forming unit–erythroid; and CFU-GEMM, CFU-granulocytic, erythroid, macrophage, megakaryocytic. The percentage of each colony type is calculated as the mean ± SEM (standard error of the mean) (n = 2-4).
Progenitor content was measured by enumerating the colony forming units (CFUs) from equal numbers of phenotypically distinct subpopulations isolated from lineage-depleted samples of human CB. The number of each type of CFU was determined from plating conditions outlined in “Materials and methods” and identified according to standard protocols.