Effects of all-trans retinoic acid on mast cell colony growth by CD34+ c-kit+ cord blood cells
| Stimuli . | Number of Mast Cell Colonies . | |||
|---|---|---|---|---|
| 1 wk . | 2 wks . | 3 wks . | 4 wks . | |
| SCF | 7 | 13 | 17 | 17 |
| SCF + ATRA (10−9 mol/L) | 0 | 8 | 8 | 4 |
| SCF + ATRA (10−8 mol/L) | 0 | 9 | 7 | 3 |
| SCF + ATRA (10−7 mol/L) | 0 | 2 | 1 | 0 |
| Stimuli . | Number of Mast Cell Colonies . | |||
|---|---|---|---|---|
| 1 wk . | 2 wks . | 3 wks . | 4 wks . | |
| SCF | 7 | 13 | 17 | 17 |
| SCF + ATRA (10−9 mol/L) | 0 | 8 | 8 | 4 |
| SCF + ATRA (10−8 mol/L) | 0 | 9 | 7 | 3 |
| SCF + ATRA (10−7 mol/L) | 0 | 2 | 1 | 0 |
ATRA = all-trans retinoic acid; SCF = stem cell factor.
The single CD34+ c-kit+ cord blood cells were sorted into the individual wells of a 96-well culture plate containing 100 ng/mL of SCF with or without different concentrations of ATRA, as described in “Materials and methods.” The number of cells in each well was serially counted until 4 weeks. Similar results were obtained in the other 2 experiments.